We narrowed to 5,339 results for: pcdna3.1

1. pCDNA3-SREBP-1 (A341; A1171)

   Plasmid

   #204197

   Purpose

   Mammalian expression of SREBP-1 (isoform 1)

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1a (SREBF1 Human)
   

   Tags

   E-tag and T7x2

   Expression

   Mammalian

   Mutation

   Gly to Ala 341, 1171

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. NSP2-Flag

   Plasmid

   #210344

   Purpose

   Expresses SARS-CoV-2 NSP2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. NSP4-FR-2Strep

   Plasmid

   #210316

   Purpose

   Expresses SARS-CoV-2 NSP4 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SARS-CoV-2 N-2Strep

   Plasmid

   #210376

   Purpose

   Expresses SARS-CoV-2 N protein fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-COV-2 N (N SARS-CoV-2)
   

   Tags

   2Strep

   Expression

   Mammalian

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CCM2_I432D_eGFP

   Plasmid

   #208879

   Purpose

   Express eGFP-CCM2 with the I432D mutation in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM2 (CCM2 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   mutation I432D

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CCM2_W421AD422A_eGFP

   Plasmid

   #208880

   Purpose

   Express eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM2 (CCM2 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   mutations W421A and D422A

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. CCM2_d410-444_eGFP

   Plasmid

   #208877

   Purpose

   Express eGFP-CCM2 lacking amino acids 410-444 in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM2 (CCM2 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   amino acids deletion : 410-444

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CCM2_I428S_eGFP

   Plasmid

   #208878

   Purpose

   Express eGFP-CCM2 with the I428S mutation in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM2 (CCM2 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   mutation I428S

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. STIM1-2Strep

   Plasmid

   #210361

   Purpose

   Expresses STIM1 fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   STIM1 (STIM1 Human)
   

   Tags

   2Strep

   Expression

   Mammalian

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. FKBP10-2Strep

    Plasmid

    #210357

    Purpose

    Expresses FKBP10 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    FKBP10 (FKBP10 Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. DNAJC11-2Strep

    Plasmid

    #210354

    Purpose

    Expresses DNAJC11 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    DNAJC11 (DNAJC11 Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA 3.1 - circMAN2A1

    Plasmid

    #198837

    Purpose

    This contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon two

    Depositor

    Stefan Stamm

    Article

    Arizaca Maquera et al Front Mol Neurosci. 2023 May 5;16:114107

    Insert

    circular mannosidase alpha class 2A member 1
    

    Expression

    Mammalian

    Mutation

    circular RNA composed by 2 exons

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA T7-Gnat1 Mut-3UTR

    Plasmid

    #184027

    Purpose

    Expresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGA

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Gnat1 (Gnat1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    TAG sites in the 3'-UTR are mutated to TGA

    Promoter

    CMV

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRD400

    Plasmid

    #163107

    Purpose

    Expression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_eYFP_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Expression

    Mammalian

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRD188

    Plasmid

    #163099

    Purpose

    Expression of mEos3.2_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mEos3.2_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Expression

    Mammalian

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. S113

    Plasmid

    #174551

    Purpose

    Constitutive PhCMV-driven mammalian expression vector of CDH-3-TF cassette (PhCMV-Gal4-mdm2-P2A-LD6-Rel65-pA)

    Depositor

    Bruno Correia

    Article

    Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

    Insert

    mdm2 and designed protein LD6 (MDM2 )
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA 3.1 AURKA deltaNter

    Plasmid

    #157749

    Purpose

    Expression of AURKA without Nter in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Expression

    Mammalian

    Mutation

    the first 30AA of Aurora A are lacking

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGAL4-DBD-OLIG2-Adel-IDR

    Plasmid

    #145249

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and OLIG2-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    OLIG2-Adel-IDR (OLIG2 Human)
    

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. S2 1058 AIDx no UGI

    Plasmid

    #135349

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-1058
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. S2 1058 APOBEC3 no UGI

    Plasmid

    #135350

    Purpose

    Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-rAPOBEC3-ID-1058
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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