We narrowed to 8,178 results for: gnal

1. GFP-Tac(5A)

   Plasmid

   #162493

   Purpose

   Expresses GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   Tac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The five threonine residues are mutated into alan…

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPICZ-aC-VTS-US

   Plasmid

   #160425

   Purpose

   Expression of c-luciferase from sp. Vargula tsujii (USA)

   Depositor

   Todd Oakley

   Article

   Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

   Insert

   luciferase
   

   Use

   Luciferase

   Tags

   6x Histidine tag, Yeast alpha secretion factor, c…

   Expression

   Yeast

   Mutation

   See depositor comments below

   Promoter

   AOX

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPICZ-aC-MSVU-BZ

   Plasmid

   #160426

   Purpose

   Expression of c-luciferase from sp. Maristella sp. SVU (Belize)

   Depositor

   Todd Oakley

   Article

   Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

   Insert

   luciferase
   

   Use

   Luciferase

   Tags

   6x Histidine tag, Yeast alpha secretion factor, c…

   Expression

   Yeast

   Mutation

   See depositor comments below

   Promoter

   AOX

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-CreERT2 sgAdam10 v3

   Plasmid

   #158047

   Purpose

   lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Adam10. NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   Adam10 sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. HBT-CPK10ac-Flag

   Plasmid

   #107427

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK10 (CDPK1 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. HBT-CPK10-Flag

   Plasmid

   #107428

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK10 (CDPK1 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HBT-CPK30ac-Flag

   Plasmid

   #107429

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK30 (CPK30 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HBT-CPK30-Flag

   Plasmid

   #107430

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK30 (CPK30 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-mCherry-mCherry-FLARE-CKAR

   Plasmid

   #123345

   Purpose

   Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   mCherry-mCherry-FLARE-CKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Cerulean3-Cerulean3-FLARE-AKAR

    Plasmid

    #123335

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-AKAR
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. LIC-Z-delCry2

    Plasmid

    #153545

    Purpose

    this is the non-clustering version of LIC-Z tagged with mcherry

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    with Cry2 sequence deleted from LIC-Z

    Promoter

    CMV

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRC/CMV-TYK2VF/PA-VSV

    Plasmid

    #139351

    Purpose

    Expression of the double mutant of TYK2-V678F-P1104A

    Depositor

    Sandra Pellegrini

    Article

    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-V678F-P1104A

    Promoter

    CMV

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MEN2A-MAC

    Plasmid

    #139634

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    MEN2A (RET Human)
    

    Use

    Tags

    StrepIII-HA-BirA*

    Expression

    Mammalian

    Mutation

    C634W

    Promoter

    CMV

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. GRB2-SHN

    Plasmid

    #139625

    Purpose

    StrepIII-HA-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    GRB2 (GRB2 Human)
    

    Use

    Tags

    StrepIII-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBB0238-TEV-His12

    Plasmid

    #137038

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66635.2
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. BtGRK2[D110A K220R]-mVenus

    Plasmid

    #137779

    Purpose

    Visualization of GRK2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Bovine GRK2[D110A K220R] (GRK2 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D110A K220R

    Promoter

    CMV

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. HsGRK6[L66A E514A]-sYFP2

    Plasmid

    #137781

    Purpose

    Visualization of GRK6

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Human GRK6[L66A E514A] (GRK6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L66A E514A

    Promoter

    CMV

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-HA-Flag-natT1R2 RR837-8GG

    Plasmid

    #113958

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-839, R837G and R838G substitution

    Promoter

    cmv

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. NES-PAmCherry-cPHx1

    Plasmid

    #116859

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    PAmCherry

    Expression

    Mammalian

    Mutation

    amino acids 169-329

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS_cyt-AKAR5

    Plasmid

    #114138

    Purpose

    Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    AKAR5 with C-terminal nuclear export signal (NES)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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