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pME-puro-Venus-FLAG-CD59

Plasmid

#50377

Purpose

Express Venus and FLAG-tagged human CD59, a GPI-AP in mammalian cells

Depositor

Reika Watanabe

Article

Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

Insert

Venus

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
3xFlag-HaloTag-SUZ12 HDR - pDY082

Plasmid

#171110

Purpose

Homology directed repair plasmid for generating HaloTag-SUZ12 cells

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

SUZ12 (SUZ12 Human)

Tags

HaloTag

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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RCAN1-aa1-150

Plasmid

#65412

Purpose

RCAN1 aa1-150 expression with CMV promoter. Flag tagged.

Depositor

Jeffery Molkentin

Article

Liu et al Nat Cell Biol. 2009 Feb;11(2):154-61. do

Insert

RCAN1-aa1-150 (Rcan1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

aa1-150 present, deleted 151 to end

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
pVF18

Plasmid

#229456

Purpose

Expresses IraD (aa17-130) with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (aa 17-130 only)

Tags

6xHis

Expression

Bacterial

Mutation

aa 17-130 only

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEY120

Plasmid

#223467

Purpose

sra-11(pB) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

sra-11(pB) (sra-11 Nematode)

Expression

Worm

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY101

Plasmid

#223448

Purpose

lin-11 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

lin-11 (lin-11 Nematode)

Expression

Worm

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEY102

Plasmid

#223449

Purpose

lin-11(intron 3) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

lin-11(intron 3) (lin-11 Nematode)

Expression

Worm

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEY93

Plasmid

#191086

Purpose

gpa-11 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gpa-11 (gpa-11 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-H2BC11_sgRNA

Plasmid

#183883

Purpose

pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 sgRNA spacer (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA4_no stop

Plasmid

#83866

Purpose

Gateway donor vector for IAA4 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA4 (ATAUX2-11 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pENTREGFP2

Plasmid

#22450

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

EGFP (eGFP )

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pAS161

Plasmid

#194484

Purpose

synER Cassette

Depositor

Ahmad Khalil

Article

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synER Cassette

Use

Synthetic Biology

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAS163

Plasmid

#194486

Purpose

synMR Cassette

Depositor

Ahmad Khalil

Article

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synMR Cassette

Use

Synthetic Biology

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAS160

Plasmid

#194483

Purpose

synDHBR Cassette

Depositor

Ahmad Khalil

Article

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synDHBR Cassette

Use

Synthetic Biology

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAS159

Plasmid

#194482

Purpose

synAR Cassette

Depositor

Ahmad Khalil

Article

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synAR Cassette

Use

Synthetic Biology

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAS162

Plasmid

#194485

Purpose

synGR Cassette

Depositor

Ahmad Khalil

Article

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synGR Cassette

Use

Synthetic Biology

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pENTRDsRedEx2

Plasmid

#22451

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

DsRedExpress

Use

Entry vector

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pPro33

Plasmid

#17810

Depositor

Jay Keasling

Article

Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only

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pPro18

Plasmid

#17806

Depositor

Jay Keasling

Article

Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only

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pPro24

Plasmid

#17805

Depositor

Jay Keasling

Article

Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only