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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 935 results for: RK2

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Showing: 461 - 480 of 935 results

  1. SAGA003

    Plasmid
    #240752
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 J23100 BCD_high - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240752-plasmid-map-sequence-id-483912

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  2. SAGA002

    Plasmid
    #240751
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 J23100 BCD_medium - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240751-plasmid-map-sequence-id-483911

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  3. SAGA001

    Plasmid
    #240750
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 J23100 BCD_low - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240750-plasmid-map-sequence-id-483909

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  6. pTA-Mob 2.0

    Plasmid
    #149662
    Purpose
    Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.
    Depositor
    Bogumil Karas
    Article
    Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:
    Type
    Empty backbone
    Use
    Synthetic Biology; Conjugation helper plasmid
    Expression
    Bacterial
    Available Since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    149662-plasmid-map-sequence-id-379005

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  8. pNDGG054

    Plasmid
    #231323
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG010, pOGG012, and pOGG014, NmR/KmR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    May 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231323-plasmid-map-sequence-id-471982

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  9. pNDGG003

    Plasmid
    #231316
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231316-plasmid-map-sequence-id-463764

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  10. pNDGG050

    Plasmid
    #231319
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Gm-par-ELT4; GG assembly from pOGG004, pOGG009, pOGG010, pOGG012, and pOGG014, GmR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231319-plasmid-map-sequence-id-466017

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  11. pNDGG052

    Plasmid
    #231321
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Sp-par-ELT4; GG assembly from pOGG004, pNDGG002, pOGG010, pOGG012, and pOGG014, SpR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    231321-plasmid-map-sequence-id-484521

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  12. pSEVAb827

    Plasmid
    #217500
    Purpose
    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb82 backbone, RK2 ori
    Depositor
    Nico Claassens
    Article
    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021
    Insert
    superfolder green fluorescent protein (sfGFP)
    Use
    Synthetic Biology
    Mutation
    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217500-plasmid-map-sequence-id-455513

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  18. pAGE2.0

    Plasmid
    #165984
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    tetracycline resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Available Since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    165984-plasmid-map-sequence-id-375867

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  19. pBGE2.0

    Plasmid
    #165987
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Tet resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    tetracycline resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Available Since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    165987-plasmid-map-sequence-id-375875

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Showing: 461 - 480 of 935 results