We narrowed to 12,864 results for: mel

1. pCFD3-ovoD1

   Plasmid

   #111142

   Purpose

   Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

   Insert

   pCFD3-ovoD1 (ovo Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hy_pMT Flag-Hel25E WT

   Plasmid

   #110123

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Tags

   Flag

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Oct. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFOSGE_DUM1H01

   Plasmid

   #71090

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   nej (nej Fly)
   

   Use

   Gateway entry vector

   Available Since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pGex4t2 with 244-458AA SUUR fragment

   Plasmid

   #112989

   Purpose

   Expresses fusion of GST and amino acids 244-458 of Drosophila SUUR

   Depositor

   Terry Orr-Weaver

   Article

   Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

   Insert

   SuUR (SuUR Fly)
   

   Expression

   Bacterial

   Mutation

   amino acids 244-458

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. KHBD00717

   Plasmid

   #39745

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG12124 (mxc Fly)
   

   Use

   Gateway donr vector

   Promoter

   none

   Available Since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

6. KHBD00327

   Plasmid

   #34450

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG18783 (Kr-h1 Fly)
   

   Use

   Gateway donor vector

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pGEX6P-1 BICC FL

   Plasmid

   #112998

   Purpose

   For protein expression and purification of full-length Drosophila BicC

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length BicC (BicC Fly)
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-white[coffee]

   Plasmid

   #84006

   Purpose

   Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4

   Depositor

   Phillip Zamore

   Article

   Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

   Insert

   A 2,080 bp fragment of the white[coffee] allele (w Fly)
   

   Use

   Unspecified

   Mutation

   A GC-to-AA mutation that creates a G589E missense…

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. KHBD00476

   Plasmid

   #39585

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG5838 (Dref Fly)
   

   Use

   Gateway donr vector

   Promoter

   none

   Available Since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

10. Hy_pMT Flag-Hel25E K91N

    Plasmid

    #110121

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    K91N mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hy_pMT Flag-Hel25E D193E

    Plasmid

    #110120

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    D193E mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMT-Pav-GFP

    Plasmid

    #24286

    Depositor

    Ron Vale

    Article

    Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

    Insert

    Pavarotti (pav Fly)
    

    Tags

    GFP

    Expression

    Insect

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

13. Hy_pMT Flag-Hel25E MUT

    Plasmid

    #110122

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    4 mutations (KKLN motif changed to RSFS)

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTNR1B-DuET

    Plasmid

    #213349

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MTNR1B (MTNR1B Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL3.7m-psMEK1tight

    Plasmid

    #89362

    Purpose

    A lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cells

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2017 Feb 24;355(6327):836-842.

    Insert

    psMEK1tight (MAP2K1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Human influenza hemagglutinin (HA) tag and pdDron…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL3.7m-psMEK1

    Plasmid

    #89361

    Purpose

    A lenti-viral vector expressing photoswitchable MEK1 in mammalian cells

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2017 Feb 24;355(6327):836-842.

    Insert

    psMEK1 (MAP2K1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Human influenza hemagglutinin (HA) tag and pdDron…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMCL-HA-MAPKK1-11A55B [S218E/S222D]

    Plasmid

    #40809

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S218E, S222D

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

18. MTNR1A-Tango

    Plasmid

    #66443

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MTNR1A (MTNR1A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET14b His Rab8a Q67L

    Plasmid

    #186014

    Purpose

    His Rab8a Q67L

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    His Rab8a Q67L (RAB8A Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Rab8WT(Flag) in pcDNA3.1neo

    Plasmid

    #46783

    Purpose

    expression of WT Rab8a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB8A (RAB8A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only