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Plasmid#85090PurposeExpresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coliDepositorInsertThe R112C/T180C variant of PCNA3 fused to P450 BM3
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3PromoterT7 promoterAvailable sinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mito-Cyan Nano-lantern(Ca2+)/pcDNA3
Plasmid#71345PurposeExpress Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian celDepositorInserthOTC_Cyan Nano-lantern(Ca2+)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZ-SEC61B-mCherry-FRB
Plasmid#233349PurposeStable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.DepositorInsertsUseRetroviralTagsExpressionMammalianMutationT2098L mutant of FKBP-rapamycin binding domain of…PromoterAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYI2206
Plasmid#228348PurposeConstitutive rPhlTA mutant expressionDepositorInsertphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143KPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1026
Plasmid#228299PurposeConstitutive expression of PhlTA mutant with weak promoter and terminatorDepositorInsertphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationE41GPromoterKpYPT1 promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT478
Plasmid#228286PurposeConstitutive mutant rPhlTA expression with strong promoterDepositorInsertrphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143KPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV BARK R106A p2A mCherry
Plasmid#117688PurposeCMV-iBARK(R106A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencingDepositorInsertBARKrgs R106A peptide
UseTagsHA / FLAGExpressionMammalianMutationR106APromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3
Plasmid#111923PurposeCyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μDepositorInsertCeNL(Ca2+)_19μ
UseTagsCoxVIIIx2ExpressionMammalianMutationE67D, F92W, E104D, D133E at CaMPromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3
Plasmid#111922PurposeCyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μDepositorInsertCeNL(Ca2+)_1.2μ
UseTagsCoxVIIIx2ExpressionMammalianMutationE67D, E104D, D133E at CaMPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+C3(R112C/T180C)X
Plasmid#108196PurposeExpresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coliDepositorInsertP450cam-PCNA3(R112C/T180C)-PdX fusion protein
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3); C73S/C85S (PdX)PromoterT7 promoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPD3cC
Plasmid#86972PurposeExpresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
UseTagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …PromoterT7 promoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
p1G108CFa
Plasmid#85089PurposeExpresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coliDepositorInsertThe G108C variant of PCNA1 fused to an FAD domain of P450 BM3
UseTagsHis6 tagExpressionBacterialMutationG108C in PCNA1, A74G/C773S/C810S in FAD domain of…PromoterT7 promoterAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT899
Plasmid#228288PurposeConstitutive mutant rPhlTA expression with strong promoterDepositorInsertrphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143KPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
POLR2A-N CRISPR pX330
Plasmid#124495PurposeA CRISPR plasmid for targeting the N-terminus coding region of human POLR2ADepositorInsertPOLR2A targeting CRISPR
UseCRISPRTagsExpressionMutationPromoterU6 promoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D BARK D110A p2A mCherry
Plasmid#117692PurposeGfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencingDepositorInsertBARKrgs D110A peptide
UseAAVTagsHA / FLAGExpressionMutationD110APromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Atg14L
Plasmid#21635DepositorInsertAtg14L (ATG14 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLINK cyclin T1 (1-280) myc (P#586)
Plasmid#14612DepositorInsertcyclin T1 (1-280) (CCNT1 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rubicon
Plasmid#21636DepositorInsertRubicon (1700021K19Rik Mouse)
UseTagsEGFPExpressionMammalianMutation*(see below)PromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
CAG FLEX BARK D110A p2A mCherry
Plasmid#117694PurposeCAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencingDepositorInsertBARKrgs D110A peptide
UseCre/LoxTagsHA / FLAGExpressionMutationD110APromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK cyclin T1 (1-300) (P#432)
Plasmid#14608DepositorInsertcyclin T1 (1-300) (CCNT1 Human)
UseTagsGSTExpressionBacterialMutationamino acids 1-300PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only