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pLX-EF1a_NKX2-1_DelCTerm_1-223AA

Plasmid

#221488

Purpose

NKX2-1 C-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPI161

Plasmid

#207439

Purpose

Shuttle vector LifeAct-mCherry

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

LifeAct-mCherry

Use

Unspecified

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-dErbB4

Plasmid

#197357

Purpose

A knock-out vector for dog ErbB4.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-bleo-ErbB4

Plasmid

#197353

Purpose

A knock-out vector for dog ErbB4.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-dErbB1

Plasmid

#197354

Purpose

A knock-out vector for dog ErbB1.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog EGFR gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-dErbB2

Plasmid

#197355

Purpose

A knock-out vector for dog ErbB2.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB2 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-dErbB3

Plasmid

#197356

Purpose

A knock-out vector for dog ErbB3.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB3 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSLS.539

Plasmid

#184958

Purpose

Test effect of a1/a2 length on editing fabH using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12

Expression

Bacterial

Mutation

fabH donor G954A

Promoter

T7/lac

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Cep41-STf

Plasmid

#182010

Purpose

CMV overexpression of SNAP-tag fast in mammalian cell lines associated to microtubules

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Cep41-STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_[Cox8a]x2_STf

Plasmid

#182003

Purpose

CMV overexpression of SNAP-tag fast in the mitochondrial matrix of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

[Cox8a]x2_STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR_STf_KDEL

Plasmid

#182004

Purpose

CMV overexpression of SNAP-tag fast in the endoplasmic reticulum of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

CalR_STf_KDEL

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_b4g-STf

Plasmid

#182005

Purpose

CMV overexpression of SNAP-tag fast in the golgi apparatus of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

b4g-STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.103

Plasmid

#184988

Purpose

Express Cas9-P2A-Eco1RT

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-P2A-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.031

Plasmid

#184968

Purpose

Test effect of extending -Eco2 a1/a2 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco2: RT and ncRNA(extended), a1/a2 length: 29

Expression

Yeast

Mutation

human codon optimized RT, a1/a2 length extended t…

Promoter

Gal7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.027

Plasmid

#184964

Purpose

Express -Eco1 RT and ncRNA in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(wt), a1/a2 length: 12

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.72

Plasmid

#184984

Purpose

Express Cas9/-Eco1 RT fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker1-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.95

Plasmid

#184986

Purpose

Express Cas9/-Eco1 RT fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker2-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.102

Plasmid

#184987

Purpose

Express -Eco1 RT-P2A-Cas9

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-P2A-Cas9

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSLS.544

Plasmid

#184961

Purpose

Test effect of a1/a2 length on editing murF using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22

Expression

Bacterial

Mutation

murF donor G1359A, a1/a2 length extended to 22 bp

Promoter

T7/lac

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.94

Plasmid

#184985

Purpose

Express -Eco1 RT/Cas9 fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-fusion_linker2-Cas9

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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