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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,260 results for: PLE;

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Showing: 4841 - 4860 of 31260 results

  1. GPR19-DuET

    Plasmid
    #213274
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR19 (GPR19 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213274-plasmid-map-sequence-id-430821

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  9. pGAPTrap-IRESMuro

    Plasmid
    #82336
    Purpose
    Base vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    IRESMuro
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    82336-plasmid-map-sequence-id-155824

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  13. pAC8_MF-P10-mCherry-pH-3C-TwinStrep (VE5621)

    Plasmid
    #139770
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter TwinStrep tag under the PH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal TwinStrep tag
    Tags
    3C - TwinStrep tag
    Promoter
    PH
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    139770-plasmid-map-sequence-id-324179

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  14. pAC8_MF-p10-mCh PH-mCh (VE5625)

    Plasmid
    #139769
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    mCherry fluorescent protein
    Expression
    Insect
    Promoter
    PH or p10
    Available Since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139769-plasmid-map-sequence-id-324116

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  15. pBrain-GFP-TACC3KDP(S558A)-shTACC3

    Plasmid
    #59357
    Purpose
    Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.
    Depositor
    Stephen Royle
    Article
    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10
    Insert
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    Use
    RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Silent mutations in amino acids 30-33 to confer s…
    Promoter
    CMV
    Available Since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    59357-plasmid-map-sequence-id-94887

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  17. pGAPTrap-mtagtdTom_IRESMuro

    Plasmid
    #82355
    Purpose
    Vector targeting the mTagtandem tomato gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    mtagTandemTomato-IRESMuro
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    82355-plasmid-map-sequence-id-156569

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Showing: 4841 - 4860 of 31260 results