Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 6,132 results for: cat.2

Showing: 4841 - 4860 of 6132 results

  1. pET28a-AviTag-SnoopLigase

    Plasmid
    #105626
    Purpose
    Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation
    Depositor
    Mark Howarth
    Article
    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3
    Insert
    AviTag-SnoopLigase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    105626-plasmid-map-sequence-id-205296

    Enlarge

  2. pET28a-HaloTag7-SnoopLigase

    Plasmid
    #105627
    Purpose
    Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7
    Depositor
    Mark Howarth
    Article
    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3
    Insert
    HaloTag7-SnoopLigase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105627-plasmid-map-sequence-id-207217

    Enlarge

  16. pJC8

    Plasmid
    #170757
    Purpose
    Co-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.
    Depositor
    Roberto Dominguez
    Article
    Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi
    Type
    Empty backbone
    Use
    Tags
    site 1- V5 tag and site 2- FLAG tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    170757-plasmid-map-sequence-id-334720

    Enlarge

  18. pMito-MtrxRFP2

    Plasmid
    #172324
    Purpose
    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)
    Depositor
    Huiwang Ai
    Article
    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.
    Insert
    mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    Use
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, SP6
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172324-plasmid-map-sequence-id-431104

    Enlarge

  20. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3

    Plasmid
    #206121
    Purpose
    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domains I, II, III and IV to to abolish divalent cation binding
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1a (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E320A, E670A, E1411A, E1707A
    Promoter
    CMV
    Available since
    Oct. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    206121-plasmid-map-sequence-id-410287

    Enlarge

Showing: 4841 - 4860 of 6132 results