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pTXTL-T7max-LuxE

Plasmid

#188309

Purpose

Expression of LuxE via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

luxE

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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MiniSOG-SpAGS-GFP

Plasmid

#185581

Purpose

for CALI

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

miniSOG-SpAGS

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2At(2nd)

Plasmid

#183087

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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LDB73

Plasmid

#185437

Purpose

NUP1 CEN URA3 vector for synthetic lethal sectoring assays and screening

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

Insert

NUP1

Expression

Yeast

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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KBB521

Plasmid

#185103

Purpose

1st 960 amino acids (C-terminal truncation) of Pom152 fused with HA and GFP

Depositor

Ken Belanger

Article

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Tags

GFP

Expression

Yeast

Mutation

POM152(1-960)-HA-GFP

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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E6SCX5

Plasmid

#163275

Purpose

Inducible expression of UniProt identifier E6SCX5

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

E6SCX5

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEPV-FLAG-DsRed2-ER-5

Plasmid

#170621

Purpose

Expresses FLAG-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

EPV-FLAG-DsRed2-ER-5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-Isce-Tol2-Tn5-neo

Plasmid

#176619

Purpose

for generating PCR templates with Tol2 repeats and Isce sites for BAC recombination

Depositor

Nathan Lawson

Article

Shih et al Development. 2021 Nov 9. pii: 273393. do

Insert

I-SceI sites, Tol2 repeats and Tn5-neo cassette

Use

Unspecified

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDH010

Plasmid

#157645

Purpose

sMHT-113 fused non-dimerizing GRX2-C37A mutant

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-GRX2_C37A
GRX2_C37A-sMHT(114-230)

Expression

Bacterial

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2005

Plasmid

#139880

Purpose

Complementation plasmid for LetAB with 2 ring (rings17) LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(delta161-747)

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2004

Plasmid

#139889

Purpose

Expression plasmid for LetAB with 6 ring (rings 123457) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta634-746)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1993

Plasmid

#139897

Purpose

Expression plasmid for LetB PLL1 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta114-128, P113G)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1999

Plasmid

#139903

Purpose

Expression plasmid for LetB PLL7 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta816-826, S815G, T827G)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2+AtAct2t

Plasmid

#170882

Purpose

Phytobrick - AtACT2t

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

AtACT2t

Expression

Plant

Mutation

426C>T to remove BbsI restriction site

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2+NOSt

Plasmid

#170885

Purpose

Phytobrick - NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

NOSt

Expression

Plant

Mutation

300C>T to remove BbsI restriction site

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD+35St

Plasmid

#170886

Purpose

Phytobrick - 35St

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

35St

Expression

Plant

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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JGI_018311

Plasmid

#158833

Purpose

Rare earth element alcohol dehydrogenases

Depositor

Joint Genome Institute

Article

Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

Insert

FAM1_3631

Use

Synthetic Biology

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMCRW2

Plasmid

#127380

Purpose

pBC with 4 repeats of 80bp DNA template

Depositor

Richard C. Willson

Article

Chavan et al Analyst. 2020 Jul 21;145(14):4942-4949.

Insert

4 repeats of 80 bp DNA template sequence

Expression

Bacterial

Promoter

lac promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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JGI_018387

Plasmid

#158871

Purpose

Rare earth element alcohol dehydrogenases

Depositor

Joint Genome Institute

Article

Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

Insert

Methyloceanibacter_5

Use

Synthetic Biology

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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JGI_018349

Plasmid

#158852

Purpose

Rare earth element alcohol dehydrogenases

Depositor

Joint Genome Institute

Article

Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

Insert

LP2A_1

Use

Synthetic Biology

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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