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pRDA_887

Plasmid

#201164

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

Depositor

John Doench

Publication

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-GFP-V5-His

Plasmid

#85621

Purpose

Expresses GFP and V5 in Drosophila.

Depositor

Kenneth Irvine

Publication

Rauskolb et al PLoS Biol. 2011 Jun;9(6):e1000624. doi:

Insert

GFP

Tags

V5, His

Expression

Insect

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHR_Gal4UAS_humanIL-2_T2A_MIP1α_PGK_mCherry

Plasmid

#85429

Purpose

Lentiviral vector for Gal4 inducible human IL-2/MIP1a with constitutive mCherry expression

Depositor

Wendell Lim

Publication

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Human IL-2 T2A MIPalpha

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLMB51

Plasmid

#40083

Depositor

Philip Poole

Publication

Tett et al Appl Environ Microbiol. 2012 Oct;78(19):

Inserts

gusA
tauR
tau promoter

Expression

Bacterial

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CMPE-ePPEplus

Plasmid

#205242

Purpose

For multiplex plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Publication

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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NINJA TC

Plasmid

#159274

Purpose

Expresses the NINJA targeting construct (inducible neoantigen expression via cumulative Cre, rtTA, doxycycline and tamoxifen).

Depositor

Nikhil Joshi

Publication

Damo et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

NINJA TC

Use

Mouse Targeting

Expression

Mammalian

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-OTI-TCRB_p2_TCRA

Plasmid

#131034

Purpose

Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

Depositor

Jeff Hammerbacher

Publication

Aksoy et al bioRxiv

Inserts

TCRB (Tcrb Mouse)
TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV/T7

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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8027_GFP-KrasG12C_2B_puro

Plasmid

#64372

Purpose

This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with puro resistance gene

Depositor

Georgios Stathopoulos

Publication

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Mutation

changed glycine 12 to cysteine

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hCtRNA_FT

Plasmid

#186715

Purpose

Fragment template for DAP array

Depositor

Xue Gao

Publication

Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

Insert

gRNA scaffold and hCtRNA

Use

CRISPR

Expression

Mammalian

Promoter

hCtRNA

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-FnCpf1

Plasmid

#129554

Purpose

Contains codon-optimized FnCpf1 and gRNA for streptomyces

Depositor

Fong Tian Wong, Mingzi M. Zhang

Publication

Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

Insert

FnCpf1

Expression

Bacterial

Promoter

rpsL(XC)-BbsI

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRSF-T20S

Plasmid

#110805

Purpose

expresses Thermoplasma acidophilium 20S proteasome in E. Coli

Depositor

Yifan Cheng

Publication

Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

Inserts

PsmB
PsmA

Tags

tev-His

Expression

Bacterial

Mutation

N2D and Q2E

Promoter

T7 and T7 promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS2+8CCerulean

Plasmid

#34937

Depositor

Amro Hamdoun

Publication

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

Cerulean

Expression

Mammalian

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
pM-ABE

Plasmid

#196292

Purpose

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

Depositor

Zhenghe Li

Publication

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV M antigenome encoding ABE in place of viral GP

Use

CRISPR

Tags

3×flag

Expression

Plant

Mutation

See depositor comments below

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-PHYTipi

Plasmid

#53283

Purpose

Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces phytoene in E. coli

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtE, idi, crtY, crtI, crtB

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRel

Plasmid

#227581

Purpose

Release plasmid with Cas9 working cassette, apramycin resistance, and pSG5 replicon

Depositor

Chengzhang Fu

Publication

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Insert

Cas9

Use

CRISPR

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_human_leucinezipperTRAIL _IRES_mCherry_PGK_tBFP

Plasmid

#85433

Purpose

Lentiviral vector for Gal4 inducible leucinezipper TRAIL with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Publication

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

leucine zipper human TRAIL secreted form

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only
pAdx-CMV-iCre-P2A-tdTomato

Plasmid

#73351

Purpose

Express iCre and tdTomato under CMV promoter

Depositor

Kazuhiro Oka

Insert

CMV-iCre-tdtomato

Use

Adenoviral

Promoter

CMV

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BBa_J72114-BBa_J72104

Plasmid

#40783

Depositor

Christopher Anderson

Publication

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ

Use

Luciferase and Synthetic Biology; Phagemid

Expression

Bacterial

Available since

Jan. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-6.24

Plasmid

#41003

Depositor

James Inglese

Publication

Cheng et al Nat Methods. 2012 Oct;9(10):937. doi: 10

Inserts

GSG-P2A-RLuc
4XCRE + Minimal promoter

Use

Luciferase

Expression

Mammalian

Available since

Nov. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti_gRNA -hMLHdn-Puro

Plasmid

#191105

Purpose

Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

Depositor

Hyongbum Kim

Publication

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

I31 silent mutation (ATA to ATC) to remove early …

Promoter

EF-1a

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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