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pcDNA5-puro-eGFP-HEC1-d80

Plasmid

#114025

Purpose

Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

Depositor

Geert Kops

Publication

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-d80-HEC1 (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 1 to 80 deleted

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-8A/S55D

Plasmid

#114032

Purpose

Inducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell lines

Depositor

Geert Kops

Publication

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eGFP-HEC1-8A/S55D (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 8A/S55D

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1-9A

Plasmid

#114023

Purpose

Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

Depositor

Geert Kops

Publication

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eGFP-HEC1 - 9A (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 9A

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBNJ26.3

Plasmid

#66876

Purpose

For CFP tagging via transposon insertion.

Depositor

Thomas Hughes

Publication

Sheridan et al BMC Biotechnol. 2004 Aug 18;4:17.

Insert

eCFP in a transposon

Use

Source for a tn7 transposon

Tags

eCFP

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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actc1b- mKate2-KDEL-pA

Plasmid

#109494

Purpose

Muscle specific zebrafish expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b- mKate2-KDEL-pA

Use

Zebrafish plasmids

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJB332

Plasmid

#115949

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Publication

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 TetR-Gly9-DDX6 (contains SapI)

Use

Synthetic Biology

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTW122

Plasmid

#115962

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Publication

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lvl0 5'-2xTetApt-mVenus-PEST

Use

Synthetic Biology

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTW324

Plasmid

#115955

Purpose

Destination Vector

Depositor

Ron Weiss

Publication

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P1 End

Use

Synthetic Biology

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTW064

Plasmid

#115951

Purpose

Destination Vector

Depositor

Ron Weiss

Publication

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 ORF Dest

Use

Synthetic Biology

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW080

Plasmid

#115928

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Publication

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/30 SGP NA (No Aptamer)

Use

Synthetic Biology

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pME-2PH-PLCd

Plasmid

#71283

Purpose

Multisite gateway entry clone for adding C-terminal fusions of human 2PH-PLC-delta

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2PH-PLC delta (Human)

Use

Gateway multisite middle entry clone

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pEarleyGate103 mmPing20F

Plasmid

#140007

Purpose

mmPing20F activation tag

Depositor

Charles Nathan Hancock

Publication

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20F

Expression

Plant

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-ChBP

Plasmid

#108892

Purpose

Multisite gateway vector for 3' tagging with a nanobody against mCherry

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

ChBP

Use

Gateway Cloning

Available since

June 23, 2018

Availability

Academic Institutions and Nonprofits only

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pRAJ11m

Plasmid

#39245

Depositor

Alfonso Jaramillo

Publication

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ11 system (no transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Rab19 cds in pGem-T Easy

Plasmid

#80504

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab19 (rab19 Zebrafish)

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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Rab5b cds in pGem-T Easy

Plasmid

#80517

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab5b (rab5b Zebrafish)

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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Rab5c cds in pGem-T Easy

Plasmid

#80518

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab5c (rab5c Zebrafish)

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-GBP

Plasmid

#67672

Purpose

Multisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

anti-GFP nanobody

Use

Gateway multisite 3' entry clone

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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RANLS-LEHD-BNES

Plasmid

#60973

Purpose

Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).

Depositor

Robert Campbell

Publication

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ddRFP A and ddRFP B

Tags

Caspase9 substrate sequence (LEHD) is after NLS s…

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
pXPR_003 sgSMARCA4 guide 1

Plasmid

#193604

Purpose

SMARCA4 knockout

Depositor

William Hahn

Publication

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgSMARCA4 guide 1 (SMARCA4 Human)

Use

Lentiviral

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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