We narrowed to 13,832 results for: Cas9

1. pMLS236

   Plasmid

   #73684

   Purpose

   SapTrap 3-site Destination vector for internal GFP tagging with embedded Cbr-unc-119 selectable marker

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   GFP + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6>XX

   Plasmid

   #87542

   Purpose

   Component 1 of 2 for U6>sgRNA cassette OSO-PCR

   Depositor

   Lionel Christiaen

   Article

   Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiGuide-Puro.3xBsmBI

   Plasmid

   #196709

   Purpose

   Backbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choice

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMLS235

   Plasmid

   #73683

   Purpose

   SapTrap 3-site Destination vector for C-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   GFP + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AP682-1

   Plasmid

   #70050

   Purpose

   Expresses TEV::eGFP::myc::3Xflag in bacteria

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

   Insert

   eGFP
   

   Use

   Tags

   TEV and myc::3Xflag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD399

   Plasmid

   #91821

   Purpose

   AID CT-tag linker donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Auxin-inducible degron (AID)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMLS360

   Plasmid

   #73687

   Purpose

   Punc-47::2xNLS-FLP-D5 Expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Punc-47::2xNLS-FLP-D5::let-858 3'-UTR
   

   Use

   Flp/frt

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Punc-47

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB8383

   Plasmid

   #126917

   Purpose

   Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   tHMG1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-SpG-P2A-mScarlet (MNW006)

   Plasmid

   #174136

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPRv2-7SK-Blast

    Plasmid

    #183620

    Purpose

    lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

    Depositor

    Bill Sugden

    Article

    Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Modified 7SK

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMLS719

    Plasmid

    #188894

    Purpose

    miniMos Arm sgRNA expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    PU6(K09B11.12)::miniMos Arm sgRNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDD375

    Plasmid

    #91825

    Purpose

    Codon-optimized mKate2 donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    mKate2-C1
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB8622

    Plasmid

    #126912

    Purpose

    Plasmid containing gRNA expression cassette targeting ADE2 in Saccharomyces cerevisiae

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    ADE2 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. U6>Ebf.774(F+E)

    Plasmid

    #59990

    Purpose

    U6 promoter driving Ebf.774 (F+E) sgRNA

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    Ebf.774 (F+E) sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ciinte.U6

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAP635-2

    Plasmid

    #99485

    Purpose

    Myc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    Myc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. YN2_1_LT84_RPL13A

    Plasmid

    #177712

    Purpose

    Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDD397

    Plasmid

    #91822

    Purpose

    AID FP-slot donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Auxin-inducible degron (AID)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LBJJ432

    Plasmid

    #117508

    Purpose

    AtEC_enh. promoter Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GGAG:pEC_enh:AATG:BsaI
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    EC1.2:EC1.1

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDD398

    Plasmid

    #91832

    Purpose

    AID NT-tag linker donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Auxin-inducible degron (AID)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAP685-2

    Plasmid

    #99490

    Purpose

    TEV::eGFP::ER::6xHis::linker::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    TEV::eGFP::RE::6Xhis::linker::3XFlag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge