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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,930 results for: bli

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Showing: 4901 - 4920 of 9930 results

  2. pJK693

    Plasmid
    #72382
    Purpose
    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)
    Depositor
    T. J. Kappock
    Insert
    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated alanine-260 to threonine
    Promoter
    T7
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72382-plasmid-map-sequence-id-134008

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  3. pCDH-Flag-hS1pr1-GFP-Neo

    Plasmid
    #203708
    Purpose
    Overexpression of FLAG-S1PR1-GFP fusion.
    Depositor
    Timothy Hla
    Insert
    Flag-hS1PR1-GFP (S1PR1 Human)
    Use
    Lentiviral
    Tags
    Flag and GFP
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available Since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    203708-plasmid-map-sequence-id-403683

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  4. CBIG-RORb

    Plasmid
    #48709
    Purpose
    bicistronic expression of RORb and EGFP
    Depositor
    Jeffrey Macklis
    Insert
    RORb (Rorb Mouse)
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CMV/beta-actin
    Available Since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48709-plasmid-map-sequence-id-70664

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  6. KSP_K406

    Plasmid
    #129771
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129771-plasmid-map-sequence-id-263572

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  7. Aiolos in Artichoke

    Plasmid
    #74452
    Purpose
    Monitor post-translational degradation of Aiolos (Lentiviral, SFFV.Aiolos-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Insert
    Aiolos (IKZF3) (IKZF3 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Promoter
    SFFV and EF1a
    Available Since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    74452-plasmid-map-sequence-id-140673

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  8. pcDNA3.3_SARS2_XBB.1.16

    Plasmid
    #201189
    Purpose
    expressing SARS-CoV-2 XBB.1.16 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XBB.1.16) (S SARS-CoV-2)
    Expression
    Mammalian
    Mutation
    E180V, T(K)478R on the basis of XBB.1.5
    Promoter
    CMV
    Available Since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    201189-plasmid-map-sequence-id-393640

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  10. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid
    #196716
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-KRAB(ZIM3)
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196716-plasmid-map-sequence-id-388040

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  11. H11-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid
    #180155
    Purpose
    td-sfGFP cre reporter with Hipp11 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180155-plasmid-map-sequence-id-355320

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  12. pNL [NlucP/HSE/Hygro] Vector

    Plasmid
    #236926
    Purpose
    Express HSE in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    HSE (HSD17B6 Human)
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Mutation
    None
    Promoter
    minP
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236926-plasmid-map-sequence-id-474661

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  13. pQXCINeo-LOX

    Plasmid
    #134761
    Purpose
    a retroviral vector to express lysyl oxidase (derived from PCR4-TOPO-LOX)
    Depositor
    Philippe Clezardin, Martine Croset
    Insert
    LOX (LOX Human)
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    134761-plasmid-map-sequence-id-265190

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  14. SmBiT-PIK3R1 Fusion Vector

    Plasmid
    #237095
    Purpose
    Express SmBiT-PIK3R1 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    PIK3R1 (PIK3R1 Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237095-plasmid-map-sequence-id-473324

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  15. PIK3R1-SmBiT Fusion Vector

    Plasmid
    #237097
    Purpose
    Express PIK3R1-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    PIK3R1 (PIK3R1 Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237097-plasmid-map-sequence-id-473326

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  16. pcDNA3.1_hSMAD4

    Plasmid
    #212710
    Purpose
    The ORF of human SMAD4, variant 1 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Insert
    Homo sapiens SMAD4 (SMAD4 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    212710-plasmid-map-sequence-id-448252

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  17. NLRX1-NanoLuc Fusion Vector

    Plasmid
    #238844
    Purpose
    Express NLRX1-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    NLRX1 (NLRX1 Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238844-plasmid-map-sequence-id-479844

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  18. pLVX-FLAG-dTomato-centrin-1

    Plasmid
    #73332
    Purpose
    Lentiviral vector encoding centrin-1 with an N-terminal FLAG and dTomato tag
    Depositor
    Manuel Thery
    Insert
    centrin-1 (CETN1 Human)
    Use
    Lentiviral
    Tags
    FLAG and dTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73332-plasmid-map-sequence-id-139043

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  20. pAAV-nEF-Con/Foff DREADD Gi-mCherry

    Plasmid
    #177673
    Purpose
    Expresses DREADD hM4D (Gi) only in Cre expressing cells and not in Flp expressing cells
    Depositor
    Karl Deisseroth
    Has Service
    AAV8
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    nEF
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177673-plasmid-map-sequence-id-348532

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Showing: 4901 - 4920 of 9930 results