We narrowed to 59,490 results for: cloning

1. pAK-EL-05

   Plasmid

   #125764

   Purpose

   Endlinker for tRNA-gRNA system when using only five guides

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   Endlinker
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFH55

   Plasmid

   #125814

   Purpose

   Level0 nSpCas9-PmCDA, Arabidopsis codon optimized

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   nSpCas9-PmCDA, Arabidopsis codon optimized
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAK-EL-02

   Plasmid

   #125761

   Purpose

   Endlinker for tRNA-gRNA system when using only two guides

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   Endlinker
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNCX2

   Plasmid

   #67148

   Purpose

   Expresses mRNA of NCX2 (Rat brain)

   Depositor

   Michela Ottolia, Kenneth Philipson

   Article

   Li et al J Biol Chem. 1994 Jul 1;269(26):17434-9.

   Insert

   NCX2
   

   Available Since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. p663-UBC-miniTurbo-V5-NEK9_IDG-K

   Plasmid

   #189873

   Purpose

   Gateway destination clone of NEK9 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   NEK9 (NEK9 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOGG009

   Plasmid

   #113983

   Purpose

   Vector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectors

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Gentamicin
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. GGE105

   Plasmid

   #120734

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Auxotrophy marker for selection after transformation in Y. lipolytica (Marker_Leu2)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   Marker_Leu2
   

   Use

   Cloning/assembly plasmid

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUS

   Plasmid

   #153416

   Purpose

   Backbone for cloning downstream of UCOE-SFFV

   Depositor

   Maki Nakayama

   Article

   Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. GGE001

   Plasmid

   #120740

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_PGM)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   P1_PGM
   

   Use

   Cloning/assembly plasmid

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. MXS_rtTA3

    Plasmid

    #62428

    Purpose

    MXS_chaining vector with rtTA3

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Reverse tetracycline transactivator
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E LAP2b

    Plasmid

    #105978

    Purpose

    3' element for Gateway cloning, lamina-associated polypeptide 2

    Depositor

    Caren Norden

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    LAP2b
    

    Expression

    Bacterial

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFH45

    Plasmid

    #125810

    Purpose

    Level0 nSpCas9-ABE7.10 base changer, human codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    nSpCas9-ABE7.10 base changer, human codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAM3110

    Plasmid

    #40247

    Depositor

    Susan Golden

    Article

    Ditty et al Microbiology. 2005 Aug;151(Pt 8):2605-13

    Insert

    attP Site
    

    Use

    Cyanobacteria cloning vector

    Promoter

    n/a

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRATIO1212

    Plasmid

    #141299

    Purpose

    Gateway cloning compatible dual fluorescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Tags

    mScarlet-I and mVenus

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. GGE080

    Plasmid

    #120774

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 3 (T3_Lip2)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    T3_Lip2
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC18PV-mini-Tn7T

    Plasmid

    #64956

    Purpose

    pUC18-mini-Tn7T with unique PvuII site in pUC18 DNA

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. BASIC_5_Amp

    Plasmid

    #68139

    Purpose

    storage vector for BASIC part: Amp

    Depositor

    Geoffrey Baldwin

    Article

    Storch et al ACS Synth Biol. 2015 Apr 16.

    Insert

    Ampicillin
    

    Expression

    Bacterial

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. GGE176

    Plasmid

    #120733

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Auxotrophy marker for selection after transformation in Y. lipolytica (Marker_Lys5)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    Marker_Lys5
    

    Use

    Cloning/assembly plasmid

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFH67

    Plasmid

    #131766

    Purpose

    Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFH34

    Plasmid

    #126008

    Purpose

    Level0 Arabidopsis AtU6-26 Pol III promoter

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    Level0 Arabidopsis AtU6-26 Pol III promoter
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge