We narrowed to 8,375 results for: construct

1. pTALE-STARVP160-C

   Plasmid

   #87536

   Purpose

   Destination vector for TALE-VP160 construction

   Depositor

   Steve Pollard

   Article

   Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology and TALEN; Tale-tf (activator)

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTALE-STARVP160-G

   Plasmid

   #87537

   Purpose

   Destination vector for TALE-VP160 construction

   Depositor

   Steve Pollard

   Article

   Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology and TALEN; Tale-tf (activator)

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTALE-STAR-KRAB-T

   Plasmid

   #87538

   Purpose

   Destination vector for TALE-KRAB construction

   Depositor

   Steve Pollard

   Article

   Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology and TALEN; Tale-tf (repressor)

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTALE-STAR-KRAB-A

   Plasmid

   #87539

   Purpose

   Destination vector for TALE-KRAB construction

   Depositor

   Steve Pollard

   Article

   Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology and TALEN; Tale-tf (repressor)

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCASCADE-fabI-gltA1-gltA2-udhA-zwf-gapAP1

   Plasmid

   #87149

   Purpose

   fabI-gltA1-gltA2-udhA-zwf-gapAP1 targeting guide RNA E. coli, Low Phosphate Induction

   Depositor

   Michael Lynch

   Insert

   fabI-gltA1-gltA2-udhA-zwf-gapAP1 guide array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lasso 1

   Plasmid

   #69935

   Purpose

   The first of the two RNA lassos required for the RNA chain construction

   Depositor

   Alfonso Jaramillo

   Article

   Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

   Insert

   Lasso 1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   TAATACGACTCACTATAGGG

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lasso 2

   Plasmid

   #69936

   Purpose

   The second of the two RNA lassos required for the RNA chain construction.

   Depositor

   Alfonso Jaramillo

   Article

   Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

   Insert

   Lasso 2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   TAATACGACTCACTATAGGG

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. AL562-2

   Plasmid

   #69937

   Purpose

   Construct targeting the PIE-target sequence.

   Depositor

   Alfonso Jaramillo

   Article

   Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

   Insert

   AL562-2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   TAATACGACTCACTATAGGG

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUCmini-iCAP-AAV.CAP-Mac

   Plasmid

   #200658

   Purpose

   Synthetic construct isolate AAV.CAP-Mac VP1 gene

   Depositor

   Viviana Gradinaru

   Article

   Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

   Insert

   Synthetic construct isolate AAV.CAP-Mac VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   7 amino acid insertion after Q588 of the AAV9 CAP…

   Promoter

   p41

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUCM-CLYBL-NGN2-BRN3A

    Plasmid

    #165597

    Purpose

    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.

    Depositor

    Carsten Bönnemann

    Article

    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…

    Available since

    March 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. DH10B E. coli aptI/gidB::Landing Pad

    Bacterial Strain

    #83036

    Purpose

    For site-specific chromosomal insertion of a target DNA sequence at an engineered landing pad site in the atpI/gidB locus.

    Depositor

    Cammie Lesser

    Article

    Reeves et al ACS Synth Biol. 2015 May 15;4(5):644-54.

    Bacterial Resistance

    Tetracycline

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pMXs-3XHA-EGFP-OMP25

    Plasmid

    #83356

    Purpose

    For tagging mitochondria with HA epitopes (HA-MITO construct)

    Depositor

    David Sabatini

    Article

    Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

    Insert

    3XHA-EGFP-OMP25
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid

    #225756

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-3XMyc-EGFP-OMP25

    Plasmid

    #83355

    Purpose

    For tagging mitochondria with Myc epitopes (Control-MITO construct)

    Depositor

    David Sabatini

    Article

    Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

    Insert

    3XMyc-EGFP-OMP25
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGTR

    Plasmid

    #63143

    Purpose

    PCR template to construct polycistronic tRNA-gRNA

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    gRNA-tRNA fusion
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXT130_TwinStrep-SUMO-ShTnsC

    Plasmid

    #135526

    Purpose

    Protein expression construct for ShTnsC

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsC
    

    Use

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXT131_TwinStrep-SUMO-ShTniQ

    Plasmid

    #135527

    Purpose

    Protein expression construct for ShTniQ

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTniQ
    

    Use

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXT129_TwinStrep-SUMO-ShTnsB

    Plasmid

    #135525

    Purpose

    Protein expression construct for ShTnsB

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB
    

    Use

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSK9065

    Plasmid

    #84345

    Purpose

    tightly controlled expression of mRFP fusion proteins in S. aureus

    Depositor

    Neville Firth

    Article

    Brzoska et al Appl Environ Microbiol. 2013 May;79(9):3

    Type

    Empty backbone

    Use

    Tags

    mRFPmars

    Expression

    Bacterial

    Mutation

    Promoter

    Pxyl/tet

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQCXIN-TetR-mCherry

    Plasmid

    #59417

    Purpose

    retroviral construct expressing the Tet repressor (TetR) tagged with mCherry

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Tet repressor protein (TetR)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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