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pSuper-gmiR-124

Plasmid

#171555

Purpose

a human miR-124-expressing plasmid

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

miR-124 (MIR124-1 Human)

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL70008

Plasmid

#50336

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

pFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pHelper_TetR/pTet-Tnpase (carbR)

Plasmid

#213911

Purpose

ATc-inducible himar1C9 transposase expression - carb/amp resistance

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

TetR/ptet ATc-inducible himar1C9 transposase

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pICH51266

Plasmid

#50267

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (1.3 kb ), 35s (Cauliflower Mosaic Virus) + 5'UTR omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pTH1-SpSrtA

Plasmid

#64987

Purpose

Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

S. pyogenes sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-81

Promoter

T7 promoter

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pAC155-pCR8-sgExpression

Plasmid

#49045

Purpose

U6-driven sgRNA expressing cassette on a gateway donor vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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IBMc090

Plasmid

#161937

Purpose

Carries engineered transposon for IBM

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2

Expression

Bacterial

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGGE001

Plasmid

#48839

Purpose

Provides the rbcS terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

rbcS terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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JP1520 PIK3CA E545K HA

Plasmid

#14571

Depositor

Joan Brugge

Article

Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

Insert

PIK3CA E545K (PIK3CA Bovine)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

E545K

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
A0A075HNX4

Plasmid

#177695

Purpose

expresses a fatty alcohol oxidase in E. coli

Depositor

Martin Engqvist

Article

Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

Insert

Long chain alcohol oxidase

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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MCV-HF

Plasmid

#32057

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-HF genome

Promoter

none from vector. Insert contains native promoter…

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mPapaya1.0

Plasmid

#52304

Purpose

An engineered monomeric Zoanthus sp. yellow fluorescent protein called mpapaya

Depositor

Robert Campbell

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

fluorescent protein mpapaya

Expression

Mammalian

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pGGF012

Plasmid

#48849

Purpose

Provides a sulfadiazine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::SulfadiazineR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition sites in SulfR removed …

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pTK_Sox9_enh_349_Citrine

Plasmid

#130594

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox9_enh_349

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pWH-DI

Plasmid

#47385

Purpose

Expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Inserts

dxs
idi

Expression

Bacterial

Promoter

PxylA

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only
p3E-U6a

Plasmid

#107593

Purpose

An entry vector with U6a promoter driving guide RNA expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

U6a promoter

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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tGFP

Plasmid

#26864

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

Truncated GFP-IRES-Puro

Expression

Bacterial

Mutation

Deletion of nt 1-36

Available Since

Nov. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pTK_Sox9_enh_283_Citrine

Plasmid

#130591

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox9_enh_283

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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JP1520 PIK3CA H1047R HA

Plasmid

#14572

Depositor

Joan Brugge

Article

Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

Insert

PIK3CA H1047R (PIK3CA Bovine)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

H1047R

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pICSL50015

Plasmid

#50318

Purpose

Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, mNEON (Branchistoma lanceolatum)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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