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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,287 results for: shRNA

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Showing: 4941 - 4960 of 12287 results

  1. pCJ1023

    Plasmid
    #228763
    Purpose
    Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Promoter
    human U6
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    228763-plasmid-map-sequence-id-455783

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  2. pCAG-nucGFP11-3xPax7gRNA

    Plasmid
    #224570
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224570-plasmid-map-sequence-id-451492

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  3. pCAG-3xGFP11-mem-3xPax7gRNA

    Plasmid
    #224571
    Purpose
    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    3xGFP11
    Use
    CRISPR
    Tags
    Membrane Localization Signal
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224571-plasmid-map-sequence-id-451493

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  11. pCRISPR/eCas9-GPIBY55

    Plasmid
    #213709
    Purpose
    To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.
    Depositor
    Qilai Huang
    Article
    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:
    Insert
    EGFP
    Use
    CRISPR
    Tags
    3XFLAG and Twin-strep-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    213709-plasmid-map-sequence-id-422949

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Showing: 4941 - 4960 of 12287 results