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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,065 results for: LEA

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Showing: 4961 - 4980 of 14065 results

  2. pAGT9054

    Plasmid
    #219430
    Purpose
    Transcriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCS
    Depositor
    Tom Schreiber
    Publication
    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000
    Insert
    LB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
    Use
    CRISPR; Moclo compatible level 1 module (position…
    Tags
    PapE-4LF2
    Expression
    Plant
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219430-plasmid-map-sequence-id-441037

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  3. nuc-GafD-mTurboID-HA

    Plasmid
    #184641
    Purpose
    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Publication
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nuc-GlycoID-HA
    Tags
    3xHA tag and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    GafD -> expressed 22-178 ; BirA* -> express…
    Promoter
    CMV
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184641-plasmid-map-sequence-id-366747

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  4. pmCer-Mcm2

    Plasmid
    #211395
    Purpose
    mammalian expression of human Mcm2 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mcm2 (MCM2 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211395-plasmid-map-sequence-id-420504

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  6. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

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  7. pPlacPbuCas13b-gRNA-T-deGFP

    Plasmid
    #184840
    Purpose
    Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Publication
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and lac promoter
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    184840-plasmid-map-sequence-id-373961

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  11. pICSL50025

    Plasmid
    #174589
    Purpose
    Level 0 C-terminal tag module carrying 6xHIS uncleavable
    Depositor
    Mark Banfield
    Insert
    6xHIS uncleavable
    Use
    Synthetic Biology
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174589-plasmid-map-sequence-id-344730

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  12. pOpen-T4 PGD

    Plasmid
    #165550
    Purpose
    T4 pyrimidine DNA glycosylase; bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T4 PGD (T4 Endonuclease V)
    Use
    Synthetic Biology
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165550-plasmid-map-sequence-id-339867

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  20. PB-NES-ZapCV2

    Plasmid
    #222950
    Purpose
    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Publication
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-ZapCV2
    Tags
    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    222950-plasmid-map-sequence-id-450583

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Showing: 4961 - 4980 of 14065 results