We narrowed to 15,981 results for: LEA

1. gRNA-his-HYB

   Plasmid

   #64333

   Purpose

   his3 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of his3 deletion gRNA cassette
   

   Expression

   Yeast

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3-Nuc-ExRai-AMPKAR

   Plasmid

   #192450

   Purpose

   ExRai-AMPKAR targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   ExRai-AMPKAR-NLS
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pN006

   Plasmid

   #188470

   Purpose

   Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   AsCas12a
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaG-30

   Plasmid

   #172754

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-30
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-CD81-(TEV)-tTA

   Plasmid

   #162598

   Purpose

   human CD81-TEV cleavage site-tTA

   Depositor

   Masaharu Somiya

   Article

   Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

   Insert

   human CD81 (CD81 Human)
   

   Tags

   TEV protease cleavage site and tTA

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21-pelB-LaM-2

   Plasmid

   #172768

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-2
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1

   Plasmid

   #129706

   Purpose

   Express cre-dependent KASH-EGFP and Gmeb1 shRNA

   Depositor

   Yi Zhang

   Article

   Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

   Insert

   KASH-EGFP
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-ABKAR

   Plasmid

   #140051

   Purpose

   Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.

   Depositor

   Jin Zhang

   Article

   Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

   Insert

   ABKAR
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-1_VNp-Uricase

   Plasmid

   #182401

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-uricase fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-uricase
   

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV E2F1 E132

    Plasmid

    #24224

    Purpose

    Mammalian expression of human E2F1 (DNA binding mutant) with HA tag

    Depositor

    Kristian Helin

    Article

    Helin et al Genes Dev. 1993 Oct . 7(10):1850-61.

    Insert

    E2F1 E132 (E2F1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

11. 3xflag-APEX2-NES

    Plasmid

    #164623

    Purpose

    Expresses a nuclear exporting signal (NES) tagged with APEX2 and 3xflag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    NES
    

    Tags

    3xFLAG and APEX2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI syn iGluSnFR

    Plasmid

    #106123

    Purpose

    Indicator for glutamate imaging (synapsin promoter)

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR
    

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Promoter

    synapsin

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDuRCC-N

    Plasmid

    #81194

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please view depositor comments below and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. BPK2301

    Plasmid

    #65778

    Purpose

    Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    St1Cas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3521 pET15b Bid p15

    Plasmid

    #8782

    Depositor

    Stanley Korsmeyer

    Article

    Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

    Insert

    Bid p15 (Bid Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

16. MSCV-mNINJ1-K45Q-GFP

    Plasmid

    #208777

    Purpose

    MSCV vector expressing K45Q mouse NINJ1

    Depositor

    Petr Broz

    Article

    Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

    Insert

    NINJ1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Mutation

    Change Lys 45 to Gln (K45Q)

    Available Since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Dlx-FLEX-hM4Di-mCherry

    Plasmid

    #209701

    Purpose

    Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner

    Depositor

    Peer Wulff

    Article

    Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

    Insert

    hM4D(Gi)
    

    Use

    AAV

    Tags

    mCherry

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSin-GFP-Fg

    Plasmid

    #174307

    Purpose

    Lentiviral expression of GFP

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    GFP (eGFP Aequorea victoria)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. OVA-miSFIT-17-T

    Plasmid

    #124677

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTU398

    Plasmid

    #178980

    Purpose

    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    gRAMP
    

    Tags

    Strep-Tag II SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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