We narrowed to 3,305 results for: FAS

1. pFastBac1-Clover4-LUMABS-S1 RBD

   Plasmid

   #183491

   Purpose

   To express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.

   Depositor

   Zongwen Jin, Jun Chu

   Article

   Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

   Insert

   Clover4-LUMABS-S1 RBD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS2393-Tier1-PhCMV-p2a_fastFT

   Plasmid

   #169530

   Purpose

   Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P2A-fused fluorescent timer, fast maturation time
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS2385-Tier1-PhCMV-fastFT

   Plasmid

   #169518

   Purpose

   Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT (PhCMV-fastFT-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven fluorescent timer, fast maturation time
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. HT114_Fsyn_FAS(Cre off)_QuasAr6b_Citrine

   Plasmid

   #178825

   Purpose

   Neuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

   Depositor

   Adam Cohen

   Article

   Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

   Insert

   QuasAr6b_citrine
   

   Use

   Cre/Lox and Lentiviral

   Tags

   citrine

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFASTbac-HisGST-PTPIP51(36-470)

   Plasmid

   #170531

   Purpose

   Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 )
   

   Use

   Tags

   GST tag and His tag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac-LacI-LAP-HEC1

   Plasmid

   #114033

   Purpose

   Production of baculovirus with LacI-LAP-HEC1

   Depositor

   Geert Kops

   Article

   Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

   Insert

   LacI-LAP-HEC1 (NDC80 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   none

   Promoter

   CMV

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-ATG101-ATG13 HORMA

   Plasmid

   #99332

   Purpose

   Mammalian expression of one subcomplex from the Human ULK1 complex

   Depositor

   James Hurley

   Article

   Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

   Inserts

   ATG101 (ATG101 Human)
   ATG13 (ATG13 Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   p10 and pH

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac(His10-Lckg2a,Y394F)

   Plasmid

   #177893

   Purpose

   Baculo expression of His10 tag fused to human LckG2A

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   Lck (LCK Human)
   

   Use

   Tags

   10xHis-TEV

   Expression

   Insect

   Mutation

   G2A,Y394F

   Promoter

   Polyhedrin

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA

   Plasmid

   #37090

   Purpose

   Expresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   hChr2(H134R)-mCherry
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac Dual His6 TEV LIC cloning vector (5B)

    Plasmid

    #30122

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1 puro-humanU6-shRNA FASN

    Plasmid

    #82327

    Purpose

    used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)

    Depositor

    Elizabeth Stoll

    Insert

    shRNA to target Fatty Acid Synthase (FASN) (FASN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac Dual α1B/β2B Tubulin

    Plasmid

    #170553

    Purpose

    Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB2B (TUBB2B Human)
    

    Use

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

    Plasmid

    #130976

    Purpose

    Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    Hook3-SNAPf (HOOK3 Human)
    

    Use

    Tags

    8His-ZZ-LTLT and SNAPf

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFAST-BAC BAF155/SMARCC1-His

    Plasmid

    #177863

    Purpose

    Transfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-His

    Depositor

    Yoshihiro Izumiya

    Article

    Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

    Insert

    SMARCC1 (SMARCC1 Human)
    

    Use

    Tags

    His and His tag at C-terminus with GGGGS linker

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-FAS-TdTomato-WPRE-pA

    Plasmid

    #37092

    Purpose

    Expresses tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    TdTomato
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-FAS-GCaMP6f-WPRE

    Plasmid

    #216277

    Purpose

    AAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Expression

    Mutation

    Promoter

    human synapsin 1 (hSyn)

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac-M13-6His-KIF1C-GFP

    Plasmid

    #130975

    Purpose

    Expression of 6His-KIF1C-GFP in insect cells for protein purification.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    KIF1C-GFP (KIF1C Human)
    

    Use

    Tags

    6His and GFP

    Expression

    Insect

    Mutation

    5 silent mutations that make this construct RNAi …

    Promoter

    Polyhedrin

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET51b-His-TEV-CLIP-tag_fast

    Plasmid

    #167275

    Purpose

    Overexpression of the fast variant (E30R) of CLIP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    CLIPf-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac-His-MBP-SCov2-nsp12

    Plasmid

    #154759

    Purpose

    Expression of SARS-CoV-2 nsp12

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedron

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Pan-Neurofascin (extracellular) scFv [A12/18]

    Plasmid

    #190485

    Purpose

    Mammalian Expression of Pan-Neurofascin (extracellular) scFV. Derived from hybridoma A12/18.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant scFV (Nfasc Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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