Addgene

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pBabe EGFR insertionH

Plasmid

#32067

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Insert H at H773-V774

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pPICZ-aC-KHC-BZ

Plasmid

#160427

Purpose

Expression of c-luciferase from sp. Kornikeria hastingsi carriebowae (Belize)

Depositor

Todd Oakley

Article

Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

Insert

luciferase

Use

Luciferase

Tags

6x Histidine tag, Yeast alpha secretion factor, c…

Expression

Yeast

Mutation

Promoter

AOX

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGMC00034 (aka pMC0235)

Plasmid

#210751

Purpose

Non-targeting guide RNA

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

Non-targeting guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR Del2

Plasmid

#32065

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Delete S752-I759

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pBabe EGFR Del4(T790M)

Plasmid

#32074

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Delete L747-P753, insert S, T790M

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pBabe EGFR Del2(T790M)

Plasmid

#32075

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Delete S752-I759, T790M

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pBabe EGFR Del3(T790M)

Plasmid

#32071

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Delete L747-A750, insert P, T790M

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pCLASP_Crz1(19A)_CLASP_RGS2membrane

Plasmid

#133085

Purpose

Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

Depositor

Hana El-Samad

Article

Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

Insert

Crz1*-CLASP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

pADH1

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3/flag-pBirA(1-G78)-FRB

Plasmid

#154447

Purpose

expresses pBirA(1-G78)-FRB/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

flag-pBirA(1-G78)-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-N23PP/L28F/G51PEKN

Plasmid

#118585

Purpose

Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Use

Tags

Expression

Bacterial

Mutation

N23 mutated to P23, L28 mutated to F28, G51 mutat…

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR Del5(T790M)

Plasmid

#32069

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Delete E746-S752, insert V, T790M

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pGMC00036 (aka pMC0223)

Plasmid

#212659

Purpose

Guide RNA targeting SERPINB3

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

SERBPINB3 guide RNA (SERPINB3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pGMC00033 (aka pMC0224)

Plasmid

#210750

Purpose

Guide RNA targeting SERPINB3

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

SERBPINB3 guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pETDuet-MerTK-PTPN1

Plasmid

#197988

Purpose

Expression of MerTK Kinase Domain (wild-type) in E. coli

Depositor

Byung Il Lee

Article

Bae et al Exp Mol Med. 2022 Sep;54(9):1450-1460. d

Inserts

Tyrosine-Protein Kinase Mer (MERTK Human)
PTPN1 (PTPN1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMAL-p2x-LanCL2

Plasmid

#154192

Purpose

To express MBP-LanCL2 in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Use

Tags

Maltose binding protein (MBP)

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-anti-MIR328-3p

Plasmid

#153319

Purpose

Expresses zsGreen fused to a miR-328-3p sponge and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

zsGreen with MIR328-3p sponge

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-anti-MIR211-5p

Plasmid

#153318

Purpose

Expresses zsGreen fused to a miR-211-5p sponge and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

zsGreen with MIR211-5p sponge

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pQlinkG2-PTPIP51(236-470)

Plasmid

#170534

Purpose

Expresses Cleavable GST-tagged PTPIP51 TPR domain (236-470) in E. Coli

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Use

Tags

GST tag

Expression

Bacterial

Mutation

Promoter

T5 promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFASTbac-HisGST-PTPIP51(36-470)

Plasmid

#170531

Purpose

Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 )

Use

Tags

GST tag and His tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pQlinkH-PTPIP51(236-470)

Plasmid

#170530

Purpose

Expresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. Coli

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T5 promoter

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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