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Th-tdTomato
Plasmid#159460PurposeTargeting vector to TH gene locus for induction of P2A-fused tdTomato, with floxed PGK-puroR drug resistant cassetteDepositorInsertP2A-tdTomato
UseCre/LoxTagsP2AExpressionMammalianMutationPromoterEndogenous THAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Th-iCre
Plasmid#159459PurposeTargeting vector to TH gene locus for induction of P2A-fused iCre, with PGK-puroR drug resistant cassette flanked by FRT sequence.DepositorInsertP2A-iCre
UseCre/LoxTagsP2AExpressionMammalianMutationPromoterEndogenous THAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
TH mAb_BCN53.1.1D10_HC_Mouse IgG2a
Plasmid#182896PurposeMammalian expression of TH mouse mAb HC geneDepositorInsertanti-TH (human) heavy chain (TH Mouse)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationNoPromoterCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TH mAb_BCN53.1.1D10_HC_Rat IgG2a
Plasmid#182898PurposeMammalian expression of TH rat mAb HC geneDepositorInsertanti-TH (human) light chain (TH Rat)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationNoPromoterCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-th-sgRNA
Plasmid#65565Purposein vitro trancription of sgRNA targeting the zebrafish th locusDepositorInsertth sgRNA (th Zebrafish)
UseIn vitro rna transcriptionTagsExpressionMutationPromoterT7Available sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3E-CaaX(tH)
Plasmid#109539PurposeMultisite gateway vector for 3' tagging with the CaaX domain from HrasDepositorInsertCaaX domain from Hras
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ths pUAST-HA
Plasmid#69769PurposeThisbe (FGF8-like-1) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertThisbe (FGF8like-1)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationEncodes amino acids 1-739 of Thisbe (NP_610701.2)…Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
th-P2A-EGFP donor
Plasmid#65562PurposeIntron targeting-mediated EGFP knockin at the zebrafish th locus.DepositorInsertth-P2A-EGFP (th Zebrafish)
UseZebrafish injectionTagsExpressionMutationPromoternoneAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
TH mAb_BCN53.1.1D10_LC_Rat Ig Kappa
Plasmid#182899PurposeMammalian expression of TH rat mAb LC geneDepositorInsertanti-TH (human) light chain (TH Rat)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationNoPromoterCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-TH (TetR, HK022)
Plasmid#45988DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
TH mAb_BCN53.1.1D10_LC_Mouse Ig Kappa
Plasmid#182897PurposeMammalian expression of TH mouse mAb LC geneDepositorInsertanti-TH (human) light chain (TH Mouse)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationNoPromoterCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
th-P2A-Gal4 donor
Plasmid#65563PurposeIntron targeting-mediated Gal4 knockin at the zebrafish th locus.DepositorInsertth-P2A-Gal4 (th Zebrafish)
UseZebrafish injectionTagsExpressionMutationPromoternoneAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-NL1
Plasmid#173007PurposeFor light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.DepositorInsertDHFR-GFP-NL1
UseTagsDHFR and EGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-GluA1
Plasmid#173008PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.DepositorInsertDHFR-GFP-th-GluA1
UseLentiviralTagsGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-CaaX(tH)
Plasmid#109487PurposeMuscle specific zebrafish expression of mKate2 fused to the CaaX domain from HrasDepositorInsertactc1b-mKate2-CaaX(tH)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-CaaX(tH) in pCSDEST2
Plasmid#140890PurposeExpression of GFP-CaaX (sequence from Hras) in mammalian cells.DepositorInsertCaaX membrane targeting motif (H-ras)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1079 - pAAV TH gRNA A EF1a EGFP
Plasmid#113159PurposeAn AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporterDepositorInsertgRNA for rat TH
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermU6Available sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAHR TH-p2a-TD:Tomato (floxed selection Puro)
Plasmid#135814PurposeFluorescent reporter for knock-in of TdTomato into the TH locusDepositorInsertTH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
UseTagsp2a-TdTomatoExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only
