We narrowed to 22,129 results for: EMB

1. pEF-DEST51 GFP-PNRC1

   Plasmid

   #123295

   Purpose

   Mammalian expression of PNRC1 N-GFP

   Depositor

   Giovanni Tonon

   Article

   Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

   Insert

   PNRC1 (PNRC1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG eGFP-Oct6

   Plasmid

   #203790

   Purpose

   Competitive assay of Oct4 and Oct6 binding to DNA motifs.

   Depositor

   Thorsten Wohland

   Article

   Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

   Insert

   Pou3f1 (Pou3f1 Mouse)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. myc-Miro1 (lacking TM)

   Plasmid

   #127613

   Purpose

   Human Miro1 with the transmembrane deleted

   Depositor

   Josef Kittler

   Article

   Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

   Insert

   Miro1 (RHOT1 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Deletion of transmembrane domain

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCUP1-GFP-Pho8-406

   Plasmid

   #207006

   Purpose

   Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   PHO8
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ptAD-seq-ubi63E-Gal4-DBD

   Plasmid

   #111930

   Purpose

   Vector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.

   Depositor

   Alexander Stark

   Article

   Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

   Insert

   tAD-seq Gal4-DBD
   

   Expression

   Insect

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFASTbac-HisGST-PTPIP51(36-470)

   Plasmid

   #170531

   Purpose

   Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 )
   

   Tags

   GST tag and His tag

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti4 Blast RFP-PNRC1

   Plasmid

   #123297

   Purpose

   Mammalian expression of PNRC1 N-RFP

   Depositor

   Giovanni Tonon

   Article

   Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

   Insert

   PNRC1 (PNRC1 Human)
   

   Use

   Lentiviral

   Tags

   TurboRFP

   Expression

   Mammalian

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF-DEST51 PNRC1

   Plasmid

   #123293

   Purpose

   Expression of PNRC1 in mammalian cells

   Depositor

   Giovanni Tonon

   Article

   Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

   Insert

   PNRC1 (PNRC1 Human)
   

   Expression

   Mammalian

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH_CMV_TFAM_mKate2

   Plasmid

   #167331

   Purpose

   Expresses mKate2 fused to full length TFAM in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Lentiviral

   Tags

   mKate2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH_blast_MCS_Nard_GFP_LAMIN

    Plasmid

    #167340

    Purpose

    Expresses GFP fused to lamin A (LA) in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCVpuro-DEST

    Plasmid

    #119745

    Purpose

    Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Puromycin resistance)

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Benitez-Guijarro et al EMBO J. 2018 Aug 1;37(15). pii: embj.201

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-FLAG-puro-PTPIP51(1-470)

    Plasmid

    #170536

    Purpose

    Expresses PTPIP51(1-470) in human HeLa cells after transfection

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

13. SPLICS NU-MT P2A

    Plasmid

    #210129

    Purpose

    Detect the Nucleus-Mitochondria contact

    Depositor

    Tito Calì

    Article

    Wu et al EMBO J. 2022 Dec 15;41(24):e112006. doi:

    Insert

    SPLICS NU-MT P2A
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-Duet1-His-TEV-mCherry-OPTN WT

    Plasmid

    #190191

    Purpose

    Plasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    optineurin (OPTN Human)
    

    Tags

    6xHis, TEV, and mCherry

    Expression

    Bacterial

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti GW V5 Eco/Dam mse EMD

    Plasmid

    #182675

    Purpose

    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.

    Depositor

    Bas van Steensel

    Article

    Amendola et al EMBO Rep. 2015 May;16(5):610-7. doi: 10.

    Insert

    Emerin (Emd Mouse)
    

    Use

    Lentiviral

    Tags

    E. Coli Dam and V5

    Expression

    Mammalian

    Promoter

    HSP

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. EHD2-T72A-mEGFP

    Plasmid

    #45934

    Purpose

    dominant-negative EHD2 mutant

    Depositor

    Ari Helenius

    Article

    Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

    Insert

    Eps-15 homology domain-containing protein2 (EHD2 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    T 72 changed to A (T72A)

    Promoter

    CMV

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-hPCM2w/b delta6

    Plasmid

    #47588

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2w/b delta6 (ATP2B2 Human)
    

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Mutation

    6 C-terminal AA deleted (needed for PDZ binding)

    Promoter

    CMV

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. EHD2-T72A-mCherry

    Plasmid

    #45935

    Purpose

    dominant-negative EHD2 mutant

    Depositor

    Ari Helenius

    Article

    Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

    Insert

    Eps-15 homology domain-containing protein2 (EHD2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T 72 changed to A (T72A)

    Promoter

    CMV

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS405 SEC66-VAC8-RFP

    Plasmid

    #166843

    Purpose

    Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8, SEC66 Budding Yeast)
    

    Tags

    RFP and SEC66 transmembrane domain

    Expression

    Yeast

    Mutation

    SEC66 transmembrane domain

    Promoter

    VAC8 promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS_m-AKAR5’

    Plasmid

    #114140

    Purpose

    Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    AKAR5' C-terminally tagged by KRas C tail
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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