We narrowed to 4,158 results for: SPL

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pAS2-mCALEBsplice.cyt (aa 465-566)

Plasmid

#89410

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Insert

CSPG5 (Cspg5 Mouse)

Tags

GAL4 DBD

Expression

Yeast

Mutation

aa 465-566

Promoter

ADH1

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGBT9-mCALEBsplice (aa 447-566)

Plasmid

#89405

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Insert

CSPG5 (Cspg5 Mouse)

Tags

GAL4 DBD

Expression

Yeast

Promoter

ADH1

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pTC16-dmd-1, splice form 3

Plasmid

#44694

Depositor

Phillip Newmark

Article

Chong et al Nat Commun. 2013; 4:1814. doi: 10.1038/n

Insert

Smed-dmd-1, spice form 3

Use

T/a cloning vector for dsrna generation and the g…

Available Since

May 29, 2013

Availability

Academic Institutions and Nonprofits only
pTC17-dmd-1, splice form 2

Plasmid

#44695

Depositor

Phillip Newmark

Article

Chong et al Nat Commun. 2013; 4:1814. doi: 10.1038/n

Insert

Smed-dmd-1, spice form 2

Use

T/a cloning vector for dsrna generation and the g…

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
p7013 MSCV-IP N-HAonly 45E6nonsplice

Plasmid

#44146

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV45E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Internal splice site mutated.

Promoter

MSCV LTR

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA

Plasmid

#216321

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice acceptor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-HaloDAmut-IRES-EGFP-CAAX

Plasmid

#234411

Purpose

Expresses the chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Article

Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

Insert

GPCR activation based chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199196

Purpose

Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

Plasmid

#199197

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int-BlastR

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)

Plasmid

#179008

Purpose

Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)

Plasmid

#179005

Purpose

Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)

Plasmid

#179004

Purpose

Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

Plasmid

#179002

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_PGE2mut-IRES-mCherry-CAAX

Plasmid

#251354

Purpose

Express the PGE2-insensitive control sensor GRAB_PGE2mut in mammalian cells

Depositor

Yulong Li

Article

Wang et al bioRxiv [Preprint]. 2025 Jun 3:2025.06.0

Insert

PGE2-insensitive sensor GRAB_PGE2mut (control sensor)

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5 CMV 3xFLAG XBP1 u (unspliced)

Plasmid

#250301

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of 3xFLAG-tagged XBP1 including intron-4 (26 nt)

Depositor

Thomas Gonatopoulos-Pournatzis

Insert

XBP1 (XBP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

April 29, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5 CMV 3xFLAG XBP1 s (spliced)

Plasmid

#250302

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of 3xFLAG-tagged XBP1 without intron-4 (26 nt)

Depositor

Thomas Gonatopoulos-Pournatzis

Insert

XBP1 (XBP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

April 29, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)--FLEx(loxP)-splitTVA:HA

Plasmid

#248665

Purpose

Helper virus for monosynaptic tracing; Expresses TVA and an HA tag in Cre-expressing cells under the CAG promoter. To be co-injected with Rabies Glycoprotein (G) viruses.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Insert

TVA

Use

AAV

Tags

HA

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[1-20]-N-int

Plasmid

#199210

Purpose

For ubiquitous expression of split-intein Gal4-N-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein Gal4[1-20]-N-int

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pDisplay-PGD2-1.0-IRES-mcherry

Plasmid

#214762

Purpose

Express GRAB-PGD2-1.0 sensor and membrane tethered mCherry under CMV promoter

Depositor

Yulong Li

Article

Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

Insert

GRAB-PGD2-1.0 sensor

Expression

Mammalian

Promoter

CMV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETC-PITPbeta (splice variant 1) K61A

Plasmid

#58271

Purpose

bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Tags

HIS

Expression

Bacterial

Mutation

K61A (abolishes PI binding)

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only