We narrowed to 2,492 results for: organization Addgene

1. pBAD miniSOG

   Plasmid

   #91774

   Purpose

   Bacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   mini Singlet Oxygen Generator
   

   Use

   Tags

   Hexa-Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-3XFLAG-hINO80

   Plasmid

   #44149

   Depositor

   Joan Conaway

   Article

   Chen et al J Biol Chem. 2011 Feb 8. ():.

   Insert

   hINO80 full length (INO80 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-N1-Cdc42BPB

   Plasmid

   #50759

   Purpose

   expression of EGFP tagged Cdc42BPB

   Depositor

   Naoki Mochizuki

   Article

   Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

   Insert

   Cdc42BPB (CDC42BPB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-FKBP-ICUE3-NLS

   Plasmid

   #138255

   Purpose

   FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

   Depositor

   Jin Zhang

   Article

   Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

   Insert

   FKBP-ICUE3-NLS
   

   Use

   Tags

   FKBP, Nuclear localization signal (NLS), cpVenus[…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLNCX2-mEGFP-VPS4A

   Plasmid

   #116924

   Purpose

   expresses mEGFP-VPS4A in mammalian cells

   Depositor

   Sanford Simon

   Article

   Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

   Insert

   VPS4A (VPS4A Human)
   

   Use

   Retroviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   silent mutation in position 4059 C to T

   Promoter

   CMV

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CTB

   Plasmid

   #141166

   Purpose

   Expresses recombinant Cholera Toxin B Subunit

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   CTB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No secretion signal

   Promoter

   T7

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. ML1Nmut_in_pEGFP-C1

   Plasmid

   #67796

   Purpose

   Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFP

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   ML1N*2(7Q)-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CBA-DO(FAS)-GCaMP6s

   Plasmid

   #110135

   Purpose

   Cre-off expression of GCaMP6s

   Depositor

   Bernardo Sabatini

   Article

   Markowitz et al Cell. 2018 May 10. pii: S0092-8674(18)30

   Insert

   GCaMP6s
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS CMV GAG

   Plasmid

   #234992

   Purpose

   For production of Viral like particles (VLPs) by expressing the MLV GAG protein in mammalian cells

   Depositor

   Monica Gonzalez-Magaldi, Daniel Leahy

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   GAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted polymerase coding sequence

   Promoter

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-PGK-ABI-dCas9-P2A-mcherry

    Plasmid

    #121513

    Purpose

    expression of AB1-fused-dcas9 plasmids

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    AB1 and P2A-mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1+(Zeo)-FLAG-muGFP

    Plasmid

    #166726

    Purpose

    Expresses FLAG-tagged monomeric ultrastable GFP (muGFP) in mammalian cells

    Depositor

    Daniel Scott

    Article

    Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

    Insert

    muGFP
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEMS-Tom20-Halo7Tag

    Plasmid

    #111135

    Purpose

    Tom20 with selflabeling tag, e.g. for fluorescence microscopy

    Depositor

    Karin Busch

    Article

    Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

    Insert

    Receptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)
    

    Use

    Tags

    Halo7 Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJJR83

    Plasmid

    #75028

    Purpose

    mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Article

    Ramalho et al Development. 2020 Jul 22;147(14):dev1880

    Insert

    mCherry^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized mCherry

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAV5B+longBRD4

    Plasmid

    #157792

    Purpose

    Expression of long isoform of BRD4

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    long BRD4 (BRD4 Human)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJJR82

    Plasmid

    #75027

    Purpose

    EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Article

    Ramalho et al Development. 2020 Jul 22;147(14):dev1880

    Insert

    EGFP^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized EGFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAM-DCA-muGFP

    Plasmid

    #166727

    Purpose

    AAV, encodes monomeric ultrastable GFP (muGFP)

    Depositor

    Daniel Scott

    Article

    Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

    Insert

    muGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Chicken Beta Actin

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-Lifeact-mRuby

    Plasmid

    #194339

    Purpose

    Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Insert

    Lifeact
    

    Use

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

    Plasmid

    #179586

    Purpose

    ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    NLS-NG-T2A-hSHH
    

    Use

    Piggybac

    Tags

    NeonGreen

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO CBX1-APEX2

    Plasmid

    #129642

    Purpose

    Express heterochromatin-localized CBX1-APEX2 fusion in mammalian cells

    Depositor

    Nicholas Ingolia

    Article

    Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

    Insert

    CBX1 (CBX1 Human)
    

    Use

    Tags

    1xFLAG and APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETduet+sfGFP-TetR-p300HAT

    Plasmid

    #157795

    Purpose

    Expression of sfGFP-TetR-p300HAT fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    sfGFP-TetR-p300 (EP300 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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