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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 4981 - 5000 of 66674 results

  1. mEmerald-Calreticulin-C-10

    Plasmid
    #54022
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Calreticulin (CALR Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  3. TKT

    Plasmid
    #39182
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TKT (TKT Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. Anti-GCN4 [IPI-C11L34] (Recombinant Antibody)

    Antibody
    #218104-rAb
    Purpose
    Anti-GCN4 tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to HLENEVARLKK sequence.
    Depositor
    Institute for Protein Innovation (IPI)
    Publication
    Zahnd et al J Biol Chem. 2004 Apr 30;279(18):18870-7
    Recommended applications
    Western Blot
    Reactivity
    Budding yeast
    Source species
    Rabbit
    Isotype
    IgG
    Trial size
    Not available to purchase
    Available since
    May 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Institute for Protein Innovation (IPI)

  5. Dronpa-ER-2

    Plasmid
    #57270
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ER (CALR Human)
    Tags
    Dronpa
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pX330A_D10A-1x7

    Plasmid
    #58777
    Purpose
    Expresses Cas9 nickase and gRNA
    Depositor
    Takashi Yamamoto
    Publication
    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    CBh
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58777-plasmid-map-sequence-id-192734

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  8. pVRC8400-SARS-CoV-2-RBD-XBB.1.5-AVI

    Plasmid
    #198466
    Purpose
    Expresses SARS-CoV-2 RBD protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Publication
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-XBB.1.5 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339H, R346T, L368I, S371F, S373P, S375F, T376A, …
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198466-plasmid-map-sequence-id-388113

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  9. pVRC8400-SARS-CoV-2-S2P-XBB.1.5-AVI

    Plasmid
    #198465
    Purpose
    Expresses SARS-CoV-2 S2P protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Publication
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-XBB.1.5 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198465-plasmid-map-sequence-id-388112

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  10. miniSOG-ER-5

    Plasmid
    #57766
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 448 / 473, Emission: 500 / 528
    Depositor
    Michael Davidson
    Publication
    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ER (CALR Human)
    Tags
    miniSOG
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57766-plasmid-map-sequence-id-254845

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  11. pX330A_D10A-1x6

    Plasmid
    #58776
    Purpose
    Expresses Cas9 nickase and gRNA
    Depositor
    Takashi Yamamoto
    Publication
    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    CBh
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58776-plasmid-map-sequence-id-91086

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  12. ECFP-ER-0

    Plasmid
    #55342
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Publication
    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ER (CALR Human)
    Tags
    ECFP
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Oct. 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pTFG011_Spike_Parental_D614G_3xFlag

    Plasmid
    #191571
    Purpose
    SARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.
    Depositor
    Joshua Leonard
    Publication
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    D614G mutation. Last 19 amino acids truncated.
    Promoter
    CMV
    Available since
    July 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    191571-plasmid-map-sequence-id-440477

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  17. pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187945
    Purpose
    dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpdCas9-tagRFPt-P2A-tagBFP
    Use
    Synthetic Biology; Piggybac
    Tags
    NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187945-plasmid-map-sequence-id-373705

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  20. p426_Cas9_gRNA-ARS1014a

    Plasmid
    #87396
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87396-plasmid-map-sequence-id-193439

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Showing: 4981 - 5000 of 66674 results