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pEGFP VAMP7 (102-220)

Plasmid

#42314

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Coiled Coil domain (102-220) (VAMP7 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Δ1-102

Promoter

CMV

Available Since

March 13, 2013

Availability

Academic Institutions and Nonprofits only
pWPI-LUC7L-3xFLAG

Plasmid

#201640

Purpose

Over-expression of 3xFLAG-tagged human LUC7L

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L (LUC7L Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2936

Plasmid

#144412

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOS (FOS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GSDMD-eGFP-SV40p-BlasR

Plasmid

#228254

Purpose

pLenti-CMV-GSDMD-eGFP-SV40p-BlasR plasmid for ectopic expression of human full-length GSDMD fusion with GFP

Depositor

Judy Lieberman

Article

Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

Insert

GSDMD (GSDMD Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pWPI-LUC7L3-3xFLAG

Plasmid

#201642

Purpose

Over-expression of 3xFLAG-tagged human LUC7L3

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L3 (LUC7L3 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

July 31, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Med25

Plasmid

#64771

Purpose

Mammalian protein expression of flag-tagged Med25

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25 (MED25 Human)

Tags

Flag

Expression

Mammalian

Promoter

pCMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-HA-CCND3

Plasmid

#172621

Purpose

Expresses HA-tagged cyclin D3 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_sgCtrl#1

Plasmid

#174148

Purpose

Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF1939

Plasmid

#141893

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MEIS1 (MEIS1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3 1 kb prom. + Enhancer CD274

Plasmid

#107005

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Mutation

N.B. There are two published SNPs in this region …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final

Plasmid

#107009

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-HDLBP

Plasmid

#154450

Purpose

Expression of FLAG/HA-tagged HDLBP

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

HDLBP (HDLBP Human)

Expression

Mammalian

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Esrrb

Plasmid

#71152

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Esrrb

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Estrogen related receptor, beta (Esrrb Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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TFORF0929

Plasmid

#143081

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ATOH8 (ATOH8 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pIRESh-GFPII-SM-Dlk1

Plasmid

#108932

Purpose

GFP-tagged vector expressing full length isoform of Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Tags

GFPII

Expression

Mammalian

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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C1-82 pComb3xss anti-cFos nanobody

Plasmid

#145829

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C1-82 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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IL2R/hE-cadherin-cytotail

Plasmid

#45773

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

IL2R/hE-cadherin-cytotail (IL2RA, CDH1 Human)

Expression

Mammalian

Mutation

Interleukin-2 receptor α subunit extracellular an…

Promoter

CMV

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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TOPO-GOLGA3

Plasmid

#226451

Purpose

For subcloning of human GOLGA3 promoter or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

GOLGA3 (GOLGA3 Human)

Use

Cloning vector

Mutation

No

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Gephyrin P1

Plasmid

#68816

Purpose

Flag tagged Geph mammalian expression

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

3xFLAG

Expression

Mammalian

Mutation

silent mutations on internal EcoRI site. Gephyrin…

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
HA-MBP-TEVpCS-ADGRC2 CTFdeltaTA-FLAG

Plasmid

#217718

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

CELSR2 (CELSR2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2362

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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