We narrowed to 10,558 results for: ESP

1. pHis-zfCSF3b

   Plasmid

   #65613

   Purpose

   express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli

   Depositor

   Petr Bartunek

   Article

   Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

   Insert

   Csf3b (csf3b Zebrafish)
   

   Tags

   (His)6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pLKO.1-puro-PARG shRNA

   Plasmid

   #23260

   Depositor

   Robert Sobol

   Article

   Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

   Insert

   poly(ADP-ribose) glycohydrolase shRNA
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

3. SEPTOPO

   Plasmid

   #64944

   Purpose

   source of SEP (Super Ecliptic pHluorin) sequence

   Depositor

   Jeremy Henley

   Article

   Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

   Insert

   SEP
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

4. GG-EBNA-EEA-guide1-PGK-Puro

   Plasmid

   #102904

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-guide1-PGK-Puro
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBW-m4136461

   Plasmid

   #149538

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

   Plasmid

   #191627

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBW-m4046758

   Plasmid

   #145670

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pLHCX-Flag-mPKM2(K433E)

   Plasmid

   #42514

   Depositor

   Dimitrios Anastasiou

   Article

   Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

   Insert

   Pyruvate Kinase M2 Lys433->Glu
   

   Use

   Retroviral

   Tags

   Flag

   Mutation

   Changed lysine 433 to glutamate

   Available Since

   March 19, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pRVGP-BB

   Plasmid

   #15380

   Depositor

   Stephen Smale

   Article

   Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

   Insert

   mU6-BRG/BRM shRNA (Smarca4, Smarca2 Mouse)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   April 18, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-SMVP-Cas9N-U6-gRNA TdL

    Plasmid

    #80938

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. plko.1/shMCT4 477

    Plasmid

    #99598

    Purpose

    Knockdown of MCT4 expression

    Depositor

    Eli Bar

    Article

    Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

    Insert

    MCT4 (SLC16A3 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJET1.2-U6

    Plasmid

    #173157

    Purpose

    Single guide RNA cassette under U6 promoter

    Depositor

    Piotr Ziolkowski

    Article

    Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

    Insert

    sgRNA cassette under U6 promoter
    

    Expression

    Plant

    Promoter

    AtU6-26

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLA CMV N-Flag RALB WT

    Plasmid

    #50990

    Purpose

    Expresses RALB in mammalian cells

    Depositor

    Anna Sablina

    Article

    Simicek et al Nat Cell Biol. 2013 Oct;15(10):1220-30.

    Insert

    RALB (RALB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCDNAIIIB rhoA WT

    Plasmid

    #61631

    Purpose

    mammalian expression of rhoA

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    rhoA (RHOA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

15. -4200GAMT-P1-pGL4

    Plasmid

    #26804

    Depositor

    Sam Lee

    Article

    Ide et al Mol Cell. 2009 Nov 13. 36(3):379-92.

    Insert

    Guanidinoacetate N-methyltransferase (GAMT Human)
    

    Use

    Luciferase

    Available Since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pTXB3 AurA

    Plasmid

    #68270

    Purpose

    bacterial expression of human AurA

    Depositor

    Stefano Ferrari

    Article

    Krystyniak et al Oncogene. 2006 Jan 19;25(3):338-48.

    Insert

    AurA (AURKA Human)
    

    Tags

    Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    See depositor comment below

    Promoter

    T7

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-TRE-DIO-eYFP-f

    Plasmid

    #104057

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequence

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP-f
    

    Use

    AAV

    Promoter

    TRE

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GWF180

    Plasmid

    #133607

    Purpose

    CAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    DNCR2-VPR, NS3aH1-tagBFP-SpdCas9
    

    Expression

    Mammalian

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-WRAP53beta

    Plasmid

    #64676

    Purpose

    expresses Wild type WRAP53beta

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    WD40-encoding RNA antisense to P53 (WRAP53 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pMM2-rPMCA2a

    Plasmid

    #47762

    Purpose

    mammalian expression of rat PMCA2a

    Depositor

    John Penniston, Emanuel Strehler

    Article

    Elwess et al J Biol Chem. 1997 Jul 18;272(29):17981-6

    Insert

    PMCA2a (Atp2b2 Rat)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only