We narrowed to 32,602 results for: Eng

1. RCP83

   Plasmid

   #163466

   Purpose

   Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP

   Depositor

   Frank Albert

   Article

   Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1

   Inserts

   Promoter fragment (Sharon2012_1922)
   His3 minimal promoter
   yeGFP
   

   Expression

   Bacterial and Yeast

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFB1_hMLH1

   Plasmid

   #129426

   Purpose

   Expression human MLH1 protein using baculovirus

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Insert

   human MLH1 (MLH1 Human)
   

   Expression

   Insect

   Promoter

   Polyhedrin promoter

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVC-Ds-E1b:eGFP-Ds

   Plasmid

   #102417

   Purpose

   Fluorescent vector for screening enhancer elements in zebrafish

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   E1b:eGFP
   

   Expression

   Bacterial

   Promoter

   Zebrafish E1b minimal promoter

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-CEPT1

   Plasmid

   #119079

   Purpose

   Visualize localization of ER enzyme CEPT1, which converts diacylglycerol precursors to phosphatidylethanolamine or phosphatidylcholine

   Depositor

   Gia Voeltz

   Article

   English et al Nat Cell Biol. 2013 Feb;15(2):169-78. do

   Insert

   CEPT1 (CEPT1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQAT42

   Plasmid

   #223414

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC103-PBNeo-U6-sgRNA-Expression

   Plasmid

   #48228

   Purpose

   sgRNA expression on PiggyBac vector with Neo-selectable marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2-lyzC-Gal4-Vp16-Crys-CFP

   Plasmid

   #97144

   Purpose

   For Gal4/UAS expression system

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   Gal4-Vp16
   

   Use

   Zebrafish

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQAT38

   Plasmid

   #223410

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants select.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCool-mRbx1-His-hCul1-CTP

   Plasmid

   #29519

   Depositor

   Ning Zheng

   Insert

   Rbx1 CUL1 (CUL1, Rbx1 Human, Mouse)
   

   Tags

   GST, His (before thrombin), and Thrombin Cut Site…

   Expression

   Bacterial

   Available Since

   May 26, 2011

   Availability

   Academic Institutions and Nonprofits only

10. T2A 3xNLS GFP

    Plasmid

    #227721

    Purpose

    Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

    Depositor

    Oliver Hobert, Haosheng Sun

    Article

    Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

    Insert

    T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
    

    Use

    Cloning vector

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTK-RetE1-Citrine

    Plasmid

    #127779

    Purpose

    Enhancer reporter construct containing the chicken RetE1 enhancer driving Citrine activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    RetE1
    

    Use

    Enhancer reporter construct

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 10-4 cyclin E promoter

    Plasmid

    #8458

    Depositor

    Bob Weinberg

    Article

    Geng et al Oncogene 1996 Mar 21;12(6):1173-80.

    Insert

    human cyclin E promoter (CCNE1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    July 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQAT46

    Plasmid

    #223418

    Purpose

    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQAT37

    Plasmid

    #223409

    Purpose

    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQAT40

    Plasmid

    #223412

    Purpose

    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC150-PBLHL-4xHS-EF1a-DEST

    Plasmid

    #48234

    Purpose

    PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2

    Plasmid

    #119061

    Purpose

    Fluorescent vector with zebrafish pax3a enhancer 5; Tol2 version

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    pax3a_enh5-E1b:eGFP
    

    Expression

    Bacterial

    Promoter

    E1b minimal promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. double cut NH-donor

    Plasmid

    #83576

    Purpose

    eGFP reporter donor containing double SA cutting sites for NHEJ knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Nhej donor

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. FlagMBNL3

    Plasmid

    #96901

    Purpose

    expression of Flag-tagged human MBNL3

    Depositor

    Thomas Cooper

    Article

    Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

    Insert

    human MBNL3 cDNA (MBNL3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDonor_RB-TnV2

    Plasmid

    #213907

    Purpose

    Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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