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pFastBac-Dual-M2

Plasmid

#135584

Purpose

High eukaryotic dual protein expression shuttle vector

Depositor

Xiao-Wen Cheng

Publication

Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

Type

Empty backbone

Use

Transfer vector

Expression

Insect

Promoter

polyhedrin of AcMNPV

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET21a-MetRS-His

Plasmid

#124115

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Methionine--tRNA ligase (metG Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-TO-dCas9-P2A-HSA

Plasmid

#121936

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Publication

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

dCas9-P2A-HSA

Use

CRISPR and Lentiviral

Tags

HSA

Expression

Mammalian

Promoter

CMV-TO

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET23b-Casp6-His

Plasmid

#11823

Depositor

Guy Salvesen

Publication

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 6 (CASP6 Human)

Tags

6xHis

Expression

Bacterial

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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HA-EglN2-pcDNA3

Plasmid

#18961

Depositor

William Kaelin

Publication

Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

Insert

Egg laying Nine - 2 (EGLN2 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3.1/V5-His Snk/Plk2

Plasmid

#16015

Depositor

Wafik El-Deiry

Publication

Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

Insert

Snk/Plk2 (PLK2 Human)

Tags

V5-His

Expression

Mammalian

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pMSCVpuro-eGFP-mcGAS

Plasmid

#108675

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGAS

Depositor

Andrew Jackson, Martin Reijns

Publication

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (Cgas Mouse)

Use

Retroviral

Promoter

LTR

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCW57.1-Luciferase

Plasmid

#99283

Purpose

All-in-one lentivector with tet-inducible luciferase

Depositor

Stephen Tapscott

Publication

Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. d

Insert

Firefly luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pBY2982

Plasmid

#23220

Depositor

Ralf Baumeister

Publication

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

BirA-mCherry

Expression

Mammalian

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#126217

Purpose

Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Publication

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pXJ40 GADD34 Wt (1-674aa)

Plasmid

#75478

Purpose

mammalian expression of GADD34

Depositor

Shirish Shenolikar

Publication

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCBFRE-(mt)-luc

Plasmid

#26896

Depositor

Nicholas Gaiano

Publication

Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

Insert

CBFRE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated CBF1 sites (see note)

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pEF6-CSPG4-myc-his

Plasmid

#69037

Purpose

Expression of CSPG4 in mammalian cells

Depositor

Wensheng Wei

Publication

Yuan et al Cell Res. 2015 Feb;25(2):157-68. doi: 10

Insert

CSPG4 (CSPG4 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

none

Promoter

EF-1 alpha

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBabepuro3-p16Flag

Plasmid

#24934

Depositor

Lynda Chin

Publication

Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

Insert

p16 (CDKN2A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pETDuet-1∆HisTag_hNMT_Nef

Plasmid

#66078

Purpose

Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system

Depositor

Dieter Willbold

Publication

Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

Inserts

NMT (NMT1 Human)
Nef

Expression

Bacterial

Mutation

Deletion of first 80 amino acids

Promoter

T7-lacO

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5myc RhoA wt

Plasmid

#15899

Depositor

Alan Hall

Publication

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

RhoA (RHOA Human)

Tags

myc

Expression

Mammalian

Available since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA

Plasmid

#183776

Purpose

AAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

Depositor

Ben Deverman

Publication

Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

Insert

Cre

Use

AAV

Mutation

NA

Promoter

CAG

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT7-7 asyn A53T

Plasmid

#105727

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Publication

Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

Insert

alpha-synuclein A53T (SNCA Human)

Expression

Bacterial

Mutation

A53T

Promoter

T7

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1mychisA XDH

Plasmid

#10958

Depositor

Toren Finkel

Publication

Ohtsubo et al Circ Res. 2004 Nov 26. 95(11):1118-24.

Insert

xanthine dehydrogenase (XDH Human)

Tags

his and myc

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCSC-NGN2-IRES-GFP-T2A-Sox11

Plasmid

#90214

Purpose

To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Publication

Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

Insert

NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Mouse, Human)

Use

Lentiviral

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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