We narrowed to 8,276 results for: 11
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Plasmid#127753PurposeResearch the effect of gene orientation of transcriptionDepositorInsertRFP, CFP, Divergent Orientation
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEYT1A
Plasmid#127760PurposeResearch the effect of gene orientation of transcriptionDepositorInsertmSpinach, MG RNA aptamer in tandem orientation
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEYRC-C1A
Plasmid#127752PurposeResearch the effect of gene orientation of transcriptionDepositorInsertRFP, CFP, Convergent Orientation
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
IgH P+ VA20
Plasmid#127249PurposeIgH V-regionDepositorInsertPromoter+V-region of IgH of A20 B cell line
UseOtherPromoterIgHAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K818A 3xFLAG
Plasmid#111982PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains K818A mutation. LethalDepositorInsertHsh155 K818A 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationK818AAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R775A 3xFLAG
Plasmid#111977PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R775A mutation. LethalDepositorInsertHsh155 R775A 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationR775AAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R778A 3xFLAG
Plasmid#111979PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R778A mutation. LethalDepositorInsertHsh155 R778A 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationR778AAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 T178A R181A R182A R186A (N4A)
Plasmid#111940PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the T178A R181A R182A R186A mutationDepositorInsertHsh155 T178A R181A R182A R186A (N4A)
ExpressionBacterial and YeastMutationT178A R181A R182A R186AAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Q773A R775A R776A Q778A (C4A)
Plasmid#111946PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q773A R775A R776A Q778A mutationDepositorInsertHsh155 Q773A R775A R776A Q778A (C4A)
ExpressionBacterial and YeastMutationQ773A R775A R776A Q778AAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M87 N184X
Plasmid#92373PurposeInducible expression of M87 N184X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
ExpressionMammalianMutation1-460 bp deleted, N184XPromoterTet-responsive P tightAvailable SinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP S245X with 5'UTR
Plasmid#92376PurposeInducible expression of S245X spastin M1 and M87 in mammalian cellsDepositorAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M87 S245X
Plasmid#92374PurposeInducible expression of M87 S245X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
ExpressionMammalianMutation1-460 bp deleted, S245XPromoterTet-responsive P tightAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1 N184X
Plasmid#92371PurposeInducible expression of M1 N184X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
ExpressionMammalianMutation1-194 bp of 5'UTR deleted, N184XPromoterTet-responsive P tightAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/SP S245X with 5'UTR
Plasmid#92370Purposeexpression of 245X spastin M1 and M87 in mammalian cellsDepositorAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestMYC_RBPMS_KDR
Plasmid#65667PurposeMYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
TagsMYCExpressionMammalianMutationintroduced HindIII site to exchange FLAG-HA tag w…PromoterCMV, 2x TetAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_K100E
Plasmid#65093PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with K100E mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationchanged lysine 100 to glutamic acid (KDR backgrou…PromoterCMV, 2x TetAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_F65A
Plasmid#65092PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with F65A mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationchange phenylalanine 65 to alanine (KDR backbone)PromoterCMV, 2x TetAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
SNV-memTdTomato
Plasmid#53123Purposeretroviral vector expressing membrane TdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorInsertmemTdTomato
UseRetroviral; Expression in chicken cellsPromoterSNV (spleen necrosis virus)Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
SNV-lacZ
Plasmid#53125Purposeretroviral vector expressing lacZ, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorInsertlacZ (lacZ E.coli)
UseRetroviral; Expression in chicken cellsPromoterSNV (spleen necrosis virus)Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-HA-shift-IRES-GFP(KaganE21)
Plasmid#52004PurposeExpresses the human MAVS CDS containing an N-terminal HA tag and a frameshift mutation inserting TA at bp number 254 of the MAVS CDS . And a GFP markerDepositorInsertMAVS-Shift
UseRetroviralTagsHAExpressionMammalianMutationA 2 nucleotide insertion was introduced between t…Available SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only