We narrowed to 30,881 results for: LIS

1. 5'-BirA*-FLAG minimal CMV pCDNA5

   Plasmid

   #212046

   Purpose

   LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 5'-3xFLAG GFP minimal CMV pCDNA5

   Plasmid

   #212086

   Purpose

   LR vector for integration of GFP into N2a FRT rtTA3 expression cells

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. GB1-His-Tev-ALIX-V (348-702)

   Plasmid

   #189819

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His-Tev-ALIX-V (348-702).

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   GB1-His-Tev-ALIX-V (348-702) (PDCD6IP Human)
   

   Use

   Tags

   GB1-His-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15b I-SceI (Ctermhis) K223I

   Plasmid

   #195615

   Purpose

   Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C2694G, G2345C, G2351C, A3365T, A3366T

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b I-SceI (Ctermhis) K122I

   Plasmid

   #195614

   Purpose

   Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C2458G, A2826T, A2827T

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPG-GPD-TRPC

   Plasmid

   #190113

   Purpose

   Expression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selection

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Fungal Genet Biol. 2017 Jul;104:29-37. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBSKΔB-24xopto-TetO-no01

   Plasmid

   #174887

   Purpose

   Plasmid vector containing southern blot probe for opto-TetO in STREAMING-tag

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   24xopto-TetO
   

   Use

   Southern blot probe

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. eSpCas9-EF

   Plasmid

   #177267

   Purpose

   CRISPR vector

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLY227

   Plasmid

   #184148

   Purpose

   short crRNA generator with spacer LEA2 with WT repeat region

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Insert

   s-crRNA-LEA2-WT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Plux2

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLY232

    Plasmid

    #184149

    Purpose

    short tracrRNA generator with WT anti-repeat region

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-tracrRNA-WT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLY171

    Plasmid

    #184145

    Purpose

    sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    sgRNA-LEA2-ex3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Plux2

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLY283

    Plasmid

    #184156

    Purpose

    Reprogrammed short tracrRNA for hijacking mRNA of zntA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-tracrRNA-zntA-1034
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ265N1

    Plasmid

    #173998

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    rAPOBEC1-zCas9(D10A)-UNG
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ265O1

    Plasmid

    #173999

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    rAPOBEC1-zCas9(D10A)-rXRCC1
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FnRPR9-10

    Plasmid

    #182568

    Purpose

    Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 9-10
    

    Use

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1374A, P1376A, R1379A

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FnRPR8-10

    Plasmid

    #182570

    Purpose

    Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 8-10
    

    Use

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1374A, P1376A, R1379A

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. px459-H3.1 (Hist1h3g) sgRNA

    Plasmid

    #186930

    Purpose

    Hist1h3g sgRNA used for genomic targeting at Hist1h3g C-terminal

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    Hist1h3g sgRNA (H3c8 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX601‐mhCMV‐miniABEmax‐N3‐ E53ogRNA

    Plasmid

    #187064

    Purpose

    Npu Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    miniABEmax-Npu
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mhCMV

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. L1-AA027

    Plasmid

    #185752

    Purpose

    Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).

    Depositor

    Peter Szoevenyi

    Article

    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

    Insert

    HygR2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-NLS-QPAS1-VP16

    Plasmid

    #186187

    Purpose

    AAV vector packaging QPAS1-VP16, a component of optogenetic system for NIR light-controllable transcriptional activation

    Depositor

    Vladislav Verkhusha

    Article

    Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

    Insert

    NLS-QPAS1-VP16
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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