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We narrowed to 6,024 results for: crispr cas9 expression plasmids

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Showing: 5021 - 5040 of 6024 results

  1. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60226
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60226-plasmid-map-sequence-id-176980

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  2. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60231
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available Since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60231-plasmid-map-sequence-id-417804

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  3. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

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  7. PEA1

    Plasmid
    #176527
    Purpose
    Delivers all prime editing nickase components in a single plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CbH for Cas9, hU6 for gRNAs
    Available Since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176527-plasmid-map-sequence-id-350558

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  13. pYTP MYRF sg1

    Plasmid
    #88857
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg1 cloned into BbsI site
    Depositor
    Mahmoud Pouladi
    Insert
    dCas9VP160 and MYRF sg1 gRNA (MYRF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    88857-plasmid-map-sequence-id-179037

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  14. pYTP MYRF sg2

    Plasmid
    #88858
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg2 cloned into BbsI site
    Depositor
    Mahmoud Pouladi
    Insert
    dCas9VP160 and MYRF sg2 gRNA (MYRF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    88858-plasmid-map-sequence-id-172479

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  15. pYTP MYRF sg3

    Plasmid
    #88859
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg3 cloned into BbsI site
    Depositor
    Mahmoud Pouladi
    Insert
    dCas9VP160 and MYRF sg3 gRNA (MYRF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    88859-plasmid-map-sequence-id-172480

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  16. pYTP MYRF sg4

    Plasmid
    #88860
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg4 cloned into BbsI site
    Depositor
    Mahmoud Pouladi
    Insert
    dCas9VP160 and MYRF sg4 gRNA (MYRF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    88860-plasmid-map-sequence-id-179756

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  17. pYTP MYRF sg5

    Plasmid
    #88861
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg5 cloned into BbsI site
    Depositor
    Mahmoud Pouladi
    Insert
    dCas9VP160 and MYRF sg5 gRNA (MYRF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    88861-plasmid-map-sequence-id-172482

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  18. pYTP MYRF sg6

    Plasmid
    #88862
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg6 cloned into BbsI site
    Depositor
    Mahmoud Pouladi
    Insert
    dCas9VP160 and MYRF sg6 gRNA (MYRF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    88862-plasmid-map-sequence-id-172483

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  19. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171991-plasmid-map-sequence-id-338127

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  20. PEA1-GFP

    Plasmid
    #171993
    Purpose
    Delivers all prime editing nickase components in a single, GFP selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171993-plasmid-map-sequence-id-340552

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Showing: 5021 - 5040 of 6024 results