We narrowed to 16,081 results for: nes
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CRISPRoff-mScarletI
Plasmid#217783PurposeExpresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencingDepositorInsertCRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
Tags2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletIExpressionMammalianPromoterCAGAvailable SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEC3115 (pSpy0K6-P23_mKate2~*ssrA)
Plasmid#218509Purposeintegrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombinationDepositorInsertmKate2
TagsssrA degradation tagExpressionBacterialMutationDeletion of Plac_mrfp cassette, introduction of a…PromoterP23Available SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-HRAS
Plasmid#52730Purposeretroviral expression of human HRASDepositorAvailable SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDREV-1
Plasmid#26751DepositorAvailable SinceJan. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-miR29b(m4)
Plasmid#21122DepositorInsertmiR-29b-1 (MIR29B1 Human)
ExpressionMammalianMutationG to C change at nucleotide 21 of the mature miR-…Available SinceMay 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pT1QciMP
Plasmid#62216PurposeQUAS transgenesis vectorDepositorTypeEmpty backboneUseZebrafish transgenesisPromoterQUAS-E1bAvailable SinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSAP
Plasmid#62293Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance markerDepositorTypeEmpty backboneExpressionInsectAvailable SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159857PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIZ-EGFP-MYO10-HMM
Plasmid#87257PurposeExpression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).DepositorInsertMYO10 (Myo10 Mouse)
ExpressionInsectMutationMyo10 is truncated to contain aa.1-941PromoterOpIE2Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-ATXN1 FL(84Q)
Plasmid#21754DepositorInsertAtaxin-1 (ATXN1 Human)
TagsGSTExpressionMammalianMutationinsert contains a stretch of 84 Glutamines and no…PromoterCMVAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
cmamxA2-GFP
Plasmid#107197PurposeExpresses human annexin A2-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deletedDepositorInsertcmannexin A2 (ANXA2 Human)
TagsGreen Fluorescent ProteinExpressionMammalianMutationall type II Ca2+ binding sites deleted ( D161A, E…PromoterCMVAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX165-HypaCas9
Plasmid#140567PurposeExpresses HypaCas9 in mammalian cellsDepositorInsertCas9
MutationN692A M694A Q695A H698AAvailable SinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
bbCas9pluspAAA
Plasmid#82581PurposeThe plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNADepositorInsertCas9
ExpressionBacterialPromoterT7Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCV
Plasmid#62659PurposeMammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancerDepositorInsertFLPo
ExpressionMammalianAvailable SinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBDR
Plasmid#62663PurposeDonor vector for tdTomato knock-in via landing padDepositorInserttdTomato
UseCre/LoxAvailable SinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
AiP12045-pAAV-3xSP10ins-AiE0359h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2045)
Plasmid#163504PurposeAiE0359h is an enhancer sequence designed to drive AAV-mediated transgene expression in Pvalb+ cortical interneurons and cerebellar Purkinje cellsDepositorInsertSYFP2
UseAAVPromoterMinRhoAvailable SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
Plasmid#159814PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-G1-ATP8-Myc-FLAG (G418)
Plasmid#86850Purposemammalian expression of ATP8 with mitochondrial targeting sequence, Myc, and FLAG tagsDepositorInsertATP8 (ATP8 Human)
TagsATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…ExpressionMammalianMutationcodon optimizedPromoterCMVAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
EC70197
Plasmid#209458PurposeCloning protospacers for Cas9 editingDepositorInsertCas9 sgRNA
UseCRISPRPromoterTaU3Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only
