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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 5021 - 5040 of 16276 results

  4. pAH243

    Plasmid
    #121147
    Purpose
    Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast
    Depositor
    Katsunori Tanaka
    Publication
    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.
    Inserts
    humanized Streptococcus pyogenes Cas9
    gRNA expression module
    Use
    CRISPR
    Tags
    Flag
    Expression
    Yeast
    Promoter
    S.pombe rrk1 and nmt41
    Available since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    121147-plasmid-map-sequence-id-234831

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  6. pET15b-sfGO-Matryoshka-LE-LP

    Plasmid
    #100018
    Purpose
    Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers
    Depositor
    Wolf Frommer
    Publication
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    cpsfGFP-LSSmOrange
    Tags
    6x HIS tag
    Expression
    Bacterial
    Mutation
    Leu and Glu N-term linker and Leu and Pro C-term …
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100018-plasmid-map-sequence-id-194292

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  9. Nano-lantern-plasma membrane/pcDNA3

    Plasmid
    #51975
    Purpose
    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane
    Depositor
    Takeharu Nagai
    Publication
    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nano-lantern
    Tags
    HIS and Lyn
    Expression
    Mammalian
    Mutation
    S257G in Rluc8
    Available since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. HDAC6 (3GV4)

    Plasmid
    #25297
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HDAC6_42 (HDAC6 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 1109-1215
    Available since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  19. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

    Plasmid
    #218509
    Purpose
    integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination
    Depositor
    Emmanuelle Charpentier
    Publication
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mKate2
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Deletion of Plac_mrfp cassette, introduction of a…
    Promoter
    P23
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218509-plasmid-map-sequence-id-438730

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Showing: 5021 - 5040 of 16276 results