We narrowed to 7,176 results for: cas9 plasmid

1. pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #222857

   Purpose

   This plasmid codes for the Slug-KH nickase with a sg(CTG)6

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   Slug-KHD10A+sgCTG
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, ITR2 and ITR2

   Expression

   Mammalian

   Mutation

   KH variant and D10A mutation

   Promoter

   H1 and eCMV

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #222859

   Purpose

   This plasmid codes for the Slug-KH nickase with a sg(AGC)6

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   Slug-KHD10A+sgAGC
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, ITR2 and ITR2

   Expression

   Mammalian

   Mutation

   KH variant and D10A mutation

   Promoter

   H1 and eCMV

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #222858

   Purpose

   This plasmid codes for the Slug-KH nickase with a sg(CAG)6

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   Slug-KHD10A+sgCAG
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, ITR2 and ITR2

   Expression

   Mammalian

   Mutation

   KH variant and D10A mutation

   Promoter

   H1 and eCMV

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #222860

   Purpose

   This plasmid codes for the Slug-KH nickase with a sg(GCA)6

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   Slug-KHD10A+sgGCA
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, ITR2 and ITR2

   Expression

   Mammalian

   Mutation

   KH variant and D10A mutation

   Promoter

   H1 and eCMV

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAP757-1

   Plasmid

   #99493

   Purpose

   6XHis::TEV::3XFlag::linker::Myc

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   6XHis::TEV::3XFlag::linker::Myc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAP605-1

   Plasmid

   #99482

   Purpose

   Myc::TEV::3XFlag::TEV::Myc

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   Myc::TEV::3XFlag::TEV::Myc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAP686-1

   Plasmid

   #99491

   Purpose

   TEV::eGFP::ER::V5::linker::3XFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   TEV::eGFP::RE::V5::linker::3XFlag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAP640-2

   Plasmid

   #99487

   Purpose

   3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCasTet-λ

   Plasmid

   #128318

   Purpose

   Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

   Depositor

   Christopher Johnston

   Article

   Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

   Insert

   TetR and pTetR/TetO
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Constitutive expression of cas9 and anhydro-tetra…

   Promoter

   tet promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRISPseq-BFP-backbone

    Plasmid

    #85707

    Purpose

    Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.

    Depositor

    Ido Amit

    Article

    Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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