We narrowed to 7,493 results for: addgene/1000
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Plasmid#238424PurposeExpresses GRP78 Delta KDEL fused to the GST TagDepositorAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only
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LentiCRISPR-B_sgRNA_RPP20 (pAVA3546)
Plasmid#239316PurposeLentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP20DepositorInsertU6-driven sgRNA targeting RPP20 (POP7 Human)
UseCRISPR and LentiviralAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hEGFR_2b)-PGKpuro2ABFP-W
Plasmid#208432PurposeLentiviral gRNA plasmid targeting human EGFR gene, co-expression of BFP tagDepositorInsertEGFR (EGFR Human)
UseCRISPR and LentiviralExpressionMammalianMutationN/APromoterhuman U6Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hEGFR_1k)-PGKpuro2ABFP-W
Plasmid#208431PurposeLentiviral gRNA plasmid targeting human EGFR gene, co-expression of BFP tagDepositorInsertEGFR (EGFR Human)
UseCRISPR and LentiviralExpressionMammalianMutationN/APromoterhuman U6Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pL(CMVtight-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)
Plasmid#236278PurposeLentiviral vector that can express doxycycline-inducible hAqp1 with fkbp12 degron in Tet ON mammalian cells. CMVtight promoter. EGFP marker.DepositorInserthuman aquaporin 1 (AQP1 Human)
UseLentiviralTagsFlag and fkbp12ExpressionMammalianPromoterCMVtightAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR2b-ITGB1(WT)
Plasmid#215450PurposeGateway entry vector with integrin beta1 wild-type (WT)DepositorInsertITGB1 (ITGB1 Human)
UseGateway entry vectorMutationSilent mutations added to disrupt shRNA binding a…PromoternoneAvailable SinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA-TAZ-CAMTA1
Plasmid#235679PurposeExpress TAZ-CAMTA1 fusion gene in mammalian cells using the CMV promoterDepositorAvailable SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA245 CMVTO_ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry
Plasmid#231206PurposeSingle transcript cageIL-10 (mRens) + cleaveIL-6 (mRens) + ER-renin (H2M)DepositorInsertERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
ExpressionMammalianMutationL147I, R148H, I203V, L290VAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-HBEGF-mNeonGreen
Plasmid#209912PurposeTo monitor the expression of HB-EGF, the plasmid encodes a recombinant HB-EGF fused intracellularly to the mNeonGreen fluorescent proteinDepositorAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-hP53DD (TET+CTD)
Plasmid#214082PurposeMammalian expression tetramerization domain (TET) and C-terminal negative regulatory domain (CTD) of human TP53DepositorAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2- GABARAP
Plasmid#212106PurposeExpression of GABARAP split tandem protein with mTagBFP2DepositorInsertGABARAP(G116A)-mTagBFP2-GABARAP (GABARAP Entacmaea quadricolor, Human)
TagsmTagBFP2ExpressionMammalianMutationG116A (lipidation deficiency) of upstream GABARAPPromoterCMVAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(G116A)
Plasmid#212108PurposeExpression of lipidation deficient GABARAP split tandem protein with mTagBFP2DepositorInsertGABARAP(G116A)-mTagBFP2-GABARAP(G116A) (GABARAP Entacmaea quadricolor, Human)
TagsmTagBFP2ExpressionMammalianMutationG116A (lipidation deficiency) of both GABARAPsPromoterCMVAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-VIM270-pCM
Plasmid#215869PurposeThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.DepositorInserttarget site which is completely complementary to passanger strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
ExpressionMammalianAvailable SinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR160-DuET
Plasmid#213268PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPBA-DuET
Plasmid#213255PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FENEF-Sensor
Plasmid#208930Purposein vitro p38-Sensor for luminescent detection of p38-activityDepositorInsertFENEF sequence from human ATF2 with phospho site (ATF2 Human)
TagsHISx6, MBP, and SmbitExpressionBacterialPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 R241A HA pMT2
Plasmid#206111Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin bindingDepositorAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
B52_puro_gRNA_3418711_KCNB2
Plasmid#197558PurposegRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)DepositorInsertupstream KCNB2 promoter gRNA (Kcnb2 Rat)
UseGrna with puromycin selectionTagspuromycin resistance cassette under CMV promotionExpressionMammalianPromoterU6Available SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsAGO2-K663AL697Y_AC
Plasmid#148427PurposeMammalian Expression of HsAGO2-K663AL697YDepositorInsertHsAGO2-K663AL697Y (AGO2 Human)
ExpressionMammalianMutationthree silent mutations and the sequence differs i…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsAGO2-P593GA623FK663AL697Y_AC
Plasmid#148428PurposeMammalian Expression of HsAGO2-P593GA623FK663AL697YDepositorInsertHsAGO2-P593GA623FK663AL697Y (AGO2 Human)
ExpressionMammalianMutationthree silent mutations and the sequence differs i…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only