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pKIF1C-BioID2-HA

Plasmid

#130980

Purpose

Expression of HA-tagged KIF1C-BioID2

Depositor

Anne Straube

Publication

Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

Insert

KIF1C-BioID2-HA (KIF1C Human)

Tags

BioID2-HA

Expression

Mammalian

Mutation

5 silent mutations that make this construct RNAi …

Promoter

CMV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1820_pCR8-DR-RG6-SA-DR

Plasmid

#118654

Purpose

Transient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

DR-RG6-SA-DR

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6-2 SNAP-Syk tSH2

Plasmid

#113028

Purpose

for purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.

Depositor

Ron Vale

Publication

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

SNAP-Syk tSH2

Tags

SNAP

Expression

Bacterial

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSL6 - Brr2 del247 WT

Plasmid

#90256

Purpose

protein expression

Depositor

Christine Guthrie

Publication

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-U2B''-fSNAPc

Plasmid

#106988

Purpose

open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Publication

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

UB2" (SNRPB2 Human)

Tags

fSNAP

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pWT016l

Plasmid

#96857

Purpose

(d)theophylline-agRNA-GFP

Depositor

David Liu

Publication

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)theophylline-agRNA-GFP

Expression

Mammalian

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pRB38

Plasmid

#72864

Purpose

template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Publication

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

Strep - His8 - Strep -TEV tag

Expression

Insect

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pKF290

Plasmid

#72859

Purpose

template for N-terminal Strep-8xHis-Strep-TEV site tag, inducible Blasticidin resistance

Depositor

Klaus Foerstemann

Publication

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

Strep - His8 - Strep -TEV tag

Expression

Insect

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pUC18PV-mini-Tn7T

Plasmid

#64956

Purpose

pUC18-mini-Tn7T with unique PvuII site in pUC18 DNA

Depositor

Herbert Schweizer

Publication

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available since

July 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pBad_crtEBI_19

Plasmid

#64045

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Publication

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pET-22HT-MSI1(7-103)

Plasmid

#60357

Purpose

Express the RRM1 of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Publication

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-103) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains amino acids 7-103 of Msi1

Promoter

T7

Available since

Nov. 24, 2014

Availability

Academic Institutions and Nonprofits only
pRS303-pGAL-HA-TMSec66-SrtA (pKS105)

Plasmid

#50033

Purpose

Galactose-inducible expression of ER-membrane S. pyogenes SrtA in yeast

Depositor

Hidde Ploegh

Publication

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

SrtA(strep)

Tags

HA

Expression

Yeast

Promoter

pGAL

Available since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pGR-L3S2P00

Plasmid

#46005

Depositor

Christopher Voigt

Publication

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

L3S2P00 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGR-ECK120010800

Plasmid

#46010

Depositor

Christopher Voigt

Publication

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120010800 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGL3-p107 1*

Plasmid

#32389

Depositor

Julien Sage

Publication

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st E2F binding site mutation: GCG-->AAA

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pGL3-p107 1*2*

Plasmid

#32387

Depositor

Julien Sage

Publication

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st and 2nd E2F binding sites mutated: GCG-->A…

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
CTA

Plasmid

#27804

Depositor

Steven Vogel

Publication

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Cerulean Traf Amber

Expression

Mammalian

Available since

July 5, 2011

Availability

Academic Institutions and Nonprofits only
pFA6a-RGS18HIS-hphMX4

Plasmid

#26883

Depositor

Peter Kaiser

Publication

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

18xHis tag

Use

Pcr tagging yeast

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pIS1-mutant RhoA 3'UTR

Plasmid

#26090

Depositor

Bob Weinberg

Publication

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

mutant RhoA 3'UTR (RHOA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Both miR-31 binding sites in 3’ UTR mutagenized. …

Available since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only
pIS1-mutant Fzd3 3'UTR

Plasmid

#25023

Depositor

Bob Weinberg

Publication

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

mutant Fzd3 3'UTR

Use

Luciferase

Expression

Mammalian

Mutation

miR-31 binding site in 3’ UTR mutagenized from TC…

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only