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pIRES2-CMV-nAChRb2(E61C)-IRES-GFP

Plasmid

#164779

Purpose

cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

Depositor

Alexandre Mourot

Article

Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

Insert

nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)

Expression

Mammalian

Mutation

E61C

Promoter

CMV

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-I152L-GGGS-TDP43 M337V

Plasmid

#162615

Purpose

Allows for transcription of improved mVenus I152L fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Mutation

M337V

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS-TDP-43

Plasmid

#162611

Purpose

Allows for transcription of mVenus fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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eIF3f- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192241

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3f

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3f-knockin-homology arm-mAID-mClover-NeoR (EIF3F Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 ATP5O(5’UTR)-SL-FF

Plasmid

#85491

Purpose

Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of ATP5O followed by stem loop (ATP5PO Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 ATP5O(TISU)-FF

Plasmid

#85489

Purpose

Firefly luciferase under the control of ATP5O TISU

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

Full TISU element of ATP5O (ATP5PO Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

2nd and 3rd codon of luciferase were modified fro…

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pUDP123

Plasmid

#107269

Purpose

pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha

Expression

Yeast

Promoter

ScTDH3

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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px330-Apob-2

Plasmid

#162549

Purpose

sgRNA targeting Apob

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Apob (Apob Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Apob-1

Plasmid

#162548

Purpose

sgRNA targeting Apob

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Apob (Apob Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Apob-3

Plasmid

#162550

Purpose

sgRNA targeting Apob

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Apob (Apob Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Apob-4

Plasmid

#162551

Purpose

sgRNA targeting Apob

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Apob (Apob Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pdest myr-AKT2 puro

Plasmid

#223053

Purpose

myr-AKT2 gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

myr-AKT2 (Akt2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-S27A Neo

Plasmid

#223059

Purpose

METTL1-S27A gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27A (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S27A

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-S27D Neo

Plasmid

#223060

Purpose

METTL1-S27D gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27D (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S27D

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdest myr-AKT2 Neo

Plasmid

#223063

Purpose

myr-AKT2 gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

myr-AKT2 (Akt2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:CCT2_CE_pISceI

Plasmid

#223041

Purpose

Express CCT2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

CCT2 (CCT2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:CNOT_CE_pISceI

Plasmid

#223046

Purpose

Express CNOT2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

CNOT2 (CNOT2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:METTL1-S27A_CE_pISceI

Plasmid

#223048

Purpose

Express METTL1-S27A gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27A (METTL1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Mutation

S27A

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:METTL1-S27D_CE_pISceI

Plasmid

#223049

Purpose

Express METTL1-S27D gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27D (METTL1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Mutation

S27D

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:METTL1-CM_CE_pISceI

Plasmid

#223050

Purpose

Express METTL1-CM gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-CM (METTL1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Mutation

160-163 (LFPD>AFPA)

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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