We narrowed to 11,580 results for: VARS

1. sgRNA BRCA2 R2842C

   Plasmid

   #139324

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA2 R2842C using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA2 R2842C using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMS1989

   Plasmid

   #49120

   Purpose

   AAV plasmid with CAGGS promoter driving expression of iCre. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-CAGGS-iCre-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pEMS2177

   Plasmid

   #111892

   Purpose

   High copy number plasmid containing Ple344 (TUBB3 MiniPromoter) insert.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple344 (TUBB3 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Ple344 (TUBB3 MiniPromoter)

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pEMS2175

   Plasmid

   #111890

   Purpose

   High copy number plasmid containing Ple342 (TUBB3 MiniPromoter) insert.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple342 (TUBB3 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Ple342 (TUBB3 MiniPromoter)

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pEMS2176

   Plasmid

   #111891

   Purpose

   High copy number plasmid containing Ple343 (TUBB3 MiniPromoter) insert.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple343 (TUBB3 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Ple343 (TUBB3 MiniPromoter)

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pUC19-E6I4Q5

   Plasmid

   #103101

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   E6I4Q5(Cas9 coding gene from Enterococcus faecalis TX0012)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

   Plasmid

   #63886

   Purpose

   An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

   Depositor

   Brandon Harvey

   Article

   Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

   Insert

   ER-localized GCaMP3(wt)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human SYN1

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1P MUTYH_2

   Plasmid

   #160786

   Purpose

   Suppress MUTYH

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shMUTYH_2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1P MUTYH_1

   Plasmid

   #160785

   Purpose

   Suppress MUTYH

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shMUTYH_1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS1984

    Plasmid

    #49115

    Purpose

    AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple253-iCre-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PITX3

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pHH0103_CRKL-2/2

    Plasmid

    #91364

    Purpose

    Protein expression and purification of human SH3 domain construct CRKL-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CRKL-2/2 (CRKL Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti CRISPR V2 sgShmt2_1

    Plasmid

    #106314

    Purpose

    Express Cas9 and sgRNA targeting mShmt2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT2 (Shmt1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEMS2276

    Plasmid

    #111894

    Purpose

    High Copy number plasmid containing Ple346 (NEFM MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple346 (NEFM Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Ple346 (NEFM MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126777

    Purpose

    Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Syn-FLEX-rc [ChromeQ-GFP]

    Plasmid

    #153540

    Purpose

    AAV-mediated expression of ChromeQ-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeQ-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-F3UXG6

    Plasmid

    #103108

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    F3UXG6(Cas9 coding gene from Streptococcus sanguinis SK49)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19-C7TEQ6

    Plasmid

    #103092

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    C7TEQ6(Cas9 coding gene from Lactobacillus rhamnosus (strain ATCC 53103 / GG))
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Thy-Kv2.1-HA

    Plasmid

    #111541

    Purpose

    For expression of human KCNB1 in mouse brain

    Depositor

    Federico Sesti

    Article

    Yu et al J Neurosci. 2016 Oct 26;36(43):11084-110

    Insert

    thy1.2-Kv2.1-HA (KCNB1 Human)
    

    Use

    Mouse Targeting

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_CD2AP-2/3

    Plasmid

    #91391

    Purpose

    Protein expression and purification of human SH3 domain construct CD2AP-2/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CD2AP-2/3 (CD2AP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. B-SpCas9

    Plasmid

    #126760

    Purpose

    Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    amino acids 1005-1013 replaced with two glycine

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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