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p365_DG

Plasmid

#234688

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and ALFA

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

ALFA and His10-bdNEDD8

Expression

Bacterial

Mutation

No

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-Exon1-MET

Plasmid

#216535

Purpose

MET CDS with 5'UTR

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET with 5'UTR

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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RLuc_MET_95_FLuc_Pless

Plasmid

#226464

Purpose

Promoterless MET predicted IRES between Rluc and Fluc

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

Predicted MET IRES

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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RLuc_MET_95_FLuc

Plasmid

#226463

Purpose

MET predicted IRES between Rluc and Fluc

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET 5'UTR

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLz48

Plasmid

#226322

Purpose

E. coli protein expression for protein purification

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

strep-CsoS1A

Tags

Strep

Expression

Bacterial

Promoter

T7

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBz12

Plasmid

#226321

Purpose

E. coli protein expression for protein purification

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

6xHis-Rubisco

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLz92

Plasmid

#226317

Purpose

E. coli protein expression for protein purification

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

6xHis-CsoS2B

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SV40-iBlue-pA

Plasmid

#200234

Purpose

Backbone for cloning iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBSII(KSplus)-HsBGG-PTC_E

Plasmid

#146186

Purpose

Bacterial Expression of HsBGG-PTC

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

HsBGG-PTC

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGT270

Plasmid

#171878

Purpose

Start-Stop Level 2 destination vector for Synechocystis sp. PCC 6803

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Type

Empty backbone

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::ECK120015170

Plasmid

#171879

Purpose

Terminator ECK120015170 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Terminator ECK120015170

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-T7Max-UTR1-T500

Plasmid

#178418

Purpose

Empty backbone. T7Max promoter

Depositor

Kate Adamala

Article

Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7Max

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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lvl0 LEC2t

Plasmid

#153376

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

LEC2 terminator

Expression

Plant

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 APT1t

Plasmid

#153386

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

APT1 terminator

Expression

Plant

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 NOSp

Plasmid

#153375

Purpose

Plant promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

NOS promoter

Expression

Plant

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 APT1p

Plasmid

#153372

Purpose

Plant promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

APT1 promoter

Expression

Plant

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 35Sp

Plasmid

#153369

Purpose

Cauliflower mosaic virus promoter for plant expression

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

35S promoter

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 ACT2t

Plasmid

#153384

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

ACT2 terminator

Expression

Plant

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pTU-A-013

Plasmid

#124415

Purpose

Slot A, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A + terminator + spacer

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-B-013

Plasmid

#124416

Purpose

Slot B, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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