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pBbA9a-RFP

Plasmid

#126082

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23113

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGBKT7-PRP43

Plasmid

#87596

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp43

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP43 (PRP43 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBLIC-neo

Plasmid

#45198

Purpose

a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Neo selection.

Depositor

Priyamvada Rai

Article

Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only
pBbE14a-RFP

Plasmid

#126147

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pNH078

Plasmid

#42164

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Expression

Worm

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pBbB12c-GFP

Plasmid

#126127

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23151

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA11a-RFP

Plasmid

#126084

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23150

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pKFSS1_Psyn-sgRNA500

Plasmid

#149557

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn and pSyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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B3rec.zf1

Plasmid

#108354

Purpose

Zebrafish codon optimized B3 recombinase that acts on blown-out sites

Depositor

Harold Burgess

Article

Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

Insert

B3 recombinase

Use

Synthetic Biology

Mutation

Zebrafish codon optimized

Promoter

lac

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 WT

Plasmid

#87220

Purpose

Encodes Hsh155 WT for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-mCherryBb

Plasmid

#149636

Purpose

Psyn-mCherry promoter strength reporter for use in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

Psyn-mCherryBb

Tags

mCherry

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGBKT7-PRP5

Plasmid

#87592

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp5

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a+D77R/T214V

Plasmid

#66135

Purpose

Expresses the D77R/T214V mutant of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Expression

Bacterial

Mutation

D77R, T214V

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pBbS18c-RFP

Plasmid

#126201

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBbS10a-RFP

Plasmid

#126173

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23116

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB18k-GFP

Plasmid

#126137

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB14c-GFP

Plasmid

#126129

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB13c-GFP

Plasmid

#126128

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23102

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbS15a-RFP

Plasmid

#126190

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac211-RPL13A-FKBPx4

Plasmid

#104474

Purpose

Plasmid for pop-in/pop-out replacement of RPL13A with an FKBPx4-tagged version.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

RPL13Ap (RPL13A Budding Yeast)

Tags

FKBPx4-HA

Expression

Yeast

Mutation

includes the last 354 bp of RPL13A only

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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