We narrowed to 11,563 results for: Vars;

1. pEMS1984

   Plasmid

   #49115

   Purpose

   AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-Ple253-iCre-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PITX3

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pHH0103_CRKL-2/2

   Plasmid

   #91364

   Purpose

   Protein expression and purification of human SH3 domain construct CRKL-2/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   CRKL-2/2 (CRKL Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CRISPR V2 sgShmt2_1

   Plasmid

   #106314

   Purpose

   Express Cas9 and sgRNA targeting mShmt2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting SHMT2 (Shmt1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEMS2276

   Plasmid

   #111894

   Purpose

   High Copy number plasmid containing Ple346 (NEFM MiniPromoter) insert.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple346 (NEFM Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Ple346 (NEFM MiniPromoter)

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126777

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   Sniper SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   F539S, M763I, K890N

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Syn-FLEX-rc [ChromeQ-GFP]

   Plasmid

   #153540

   Purpose

   AAV-mediated expression of ChromeQ-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

   Insert

   ChromeQ-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-F3UXG6

   Plasmid

   #103108

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   F3UXG6(Cas9 coding gene from Streptococcus sanguinis SK49)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-C7TEQ6

   Plasmid

   #103092

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   C7TEQ6(Cas9 coding gene from Lactobacillus rhamnosus (strain ATCC 53103 / GG))
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Thy-Kv2.1-HA

   Plasmid

   #111541

   Purpose

   For expression of human KCNB1 in mouse brain

   Depositor

   Federico Sesti

   Article

   Yu et al J Neurosci. 2016 Oct 26;36(43):11084-110

   Insert

   thy1.2-Kv2.1-HA (KCNB1 Human)
   

   Use

   Mouse Targeting

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_CD2AP-2/3

    Plasmid

    #91391

    Purpose

    Protein expression and purification of human SH3 domain construct CD2AP-2/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CD2AP-2/3 (CD2AP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. B-SpCas9

    Plasmid

    #126760

    Purpose

    Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    amino acids 1005-1013 replaced with two glycine

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVLAD6-mmPtch1-B-NNQ-TEV-SBP

    Plasmid

    #120887

    Purpose

    BacMam vector for Ptch1-B NNQ variant expression

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    SBP

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291, N…

    Promoter

    CMV

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEMS2058

    Plasmid

    #49136

    Purpose

    AAV plasmid with CAGGS promoter driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-CAGGS-emGFP-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. mTFP1-Gamma-Tubulin-17

    Plasmid

    #55485

    Purpose

    Localization: Centrosomes, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Gamma-Tubulin (TUBG1 Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3_altPKA-Cα-mPAGFP

    Plasmid

    #114133

    Purpose

    Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    Testis only splice variant of mouse PKA catalytic subunit α that has no myristoylation site, C-terminally tagged by mPAGFP (Prkaca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDEL

    Plasmid

    #63884

    Purpose

    An AAV packaging vector that expresses ER-retained GCaMP3 under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    ER-localized GCaMP3(wt)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHH0103_CD2AP-1/3

    Plasmid

    #91398

    Purpose

    Protein expression and purification of human SH3 domain construct CD2AP-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CD2AP-1/3 (CD2AP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103_EPS8-1/1

    Plasmid

    #91254

    Purpose

    Protein expression and purification of human SH3 domain construct EPS8-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    EPS8-1/1 (EPS8 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS1958

    Plasmid

    #82569

    Purpose

    High copy number plasmid containing PR2/3:Con MiniPromoter insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    PRS2/3:Con
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pHH0103_BAIAP2L2-1/1

    Plasmid

    #91393

    Purpose

    Protein expression and purification of human SH3 domain construct BAIAP2L2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BAIAP2L2-1/1 (BAIAP2L2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge