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pMaCTag-33

Plasmid

#119986

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with FLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

FLAG

Use

Pcr template

Tags

FLAG

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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JBEI-6822

Plasmid

#84985

Purpose

For expression of pBbA5c-MevTaco-MKco-PMKco(aka 2A) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-MKco-PMKco

Expression

Bacterial

Promoter

LacUV5

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-25

Plasmid

#120004

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-19

Plasmid

#124787

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProGL2 GW

Plasmid

#80689

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Arp Schnittger

Article

Weinl et al Plant Cell. 2005 Jun;17(6):1704-22. Epub

Type

Empty backbone

Expression

Plant

Promoter

ProGL2

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rab23 DN

Plasmid

#37727

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab23 (Rab23 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S51N (dominant negative)

Promoter

GAL4

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab23 CA

Plasmid

#37728

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab23 (Rab23 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q96L (constitutive active)

Promoter

GAL4

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
Pr-delRBS-deGFP

Plasmid

#67744

Purpose

Pr promoter expressing a deGFP that is missing a ribosomal binding site

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

deGFP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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mP14H1

Plasmid

#84369

Purpose

antigen for mouse PRDM14 purification ( aa 1-231)

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14

Tags

6xHis

Expression

Bacterial

Mutation

only aa 1-231 present

Promoter

T7

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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hP14H8y

Plasmid

#84368

Purpose

antigen for human PRDM14 purification ( aa 1-243)

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Tags

6xHis

Expression

Bacterial

Mutation

contains PRDM14 AA1-243

Promoter

T7

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSL14 - Prp8 2142–2398

Plasmid

#90264

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

PRP8 (PRP8 Budding Yeast)

Tags

N-terminal 6xHis tag

Expression

Bacterial

Mutation

only Jab1 domain (aa 2143-2398)

Promoter

T7

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P03

Plasmid

#120014

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pThom77.2

Plasmid

#101646

Purpose

Encodes the fluorescent protein amFP486 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

amFP486

Tags

6XHis

Expression

Bacterial

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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T7T3-PacI-Neto1

Plasmid

#87672

Purpose

In situ probe for mouse Neto1

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Neto1 (Neto1 Mouse)

Expression

Bacterial

Mutation

partial sequence, (1244 to 2278bp of NM_144946.4)

Promoter

T7/T3

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmDCP2-V5His6

Plasmid

#22409

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

decapping protein 2, variant A (DCP2 Fly)

Use

Drosophila expression

Tags

V5-His6 and lambdaN-HA

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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p252 pPr-CREM/CMV-STOP-luc

Plasmid

#8406

Purpose

Expression of modified Cre from androgen-inducible probasin promoter and LOX-dependent conditional reporter cassette.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM/lox/luciferase

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre

Promoter

Probasin promoter, CMV

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pUAST YFP Rab2 WT

Plasmid

#58444

Purpose

expression of drosophila YFP-Rab2

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab2 (Rab2 Fly)

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pBSU6-shTom40.3/CMV-eGFP

Plasmid

#89797

Purpose

Expresses shRNA against TOM40.

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Tom40

Tags

eGFP under separate promoter

Expression

Mammalian

Promoter

U6

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-OR2-OR1-Pr-UTR1-deGFPT500 (121p)

Plasmid

#70197

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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P4U2C6T2-v1-2 (Pl-tetO1-lacI) (137p)

Plasmid

#70213

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

lacI

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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