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pEC3135 (pERASE)

Plasmid

#220306

Purpose

Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pKm-HCBE

Plasmid

#170333

Purpose

a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized CBEs

Use

CRISPR

Mutation

Cas9n-D10A

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGilesSHdI_psmyc_His6_triple red

Plasmid

#170047

Purpose

Mono-Responsive Reporter Gene Constructs for triple red

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mScarlet
COmScarlet
mRuby3

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pME-secAnxaV-NS

Plasmid

#67718

Purpose

Gateway entry clone containing secreted human AnxaV with no stop codon

Depositor

Rob Parton

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

secAnxaV-NS

Expression

Mammalian

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pGST-EGFP-GPIBY55

Plasmid

#213714

Purpose

Express the affinity sorting tag GST-EGFP- GPIBY55.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGST-EGFP-GPIDAF

Plasmid

#213713

Purpose

Express the affinity sorting tag GST-EGFP- GPIDAF.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-hTipin (pc4732)

Plasmid

#216862

Purpose

Mammalian expression vector encoding GFP tagged human Tipin

Depositor

Cristina Cardoso

Article

Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

Insert

Tipin (TIPIN Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKm-HGBE

Plasmid

#170334

Purpose

a modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized GBEs

Use

CRISPR

Mutation

Cas9n-D10A

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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mTB3-BC2 nanobody

Plasmid

#210208

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

BC2 Nanobody

Expression

Bacterial

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKm-HABE

Plasmid

#170332

Purpose

a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized ABEs

Use

CRISPR

Mutation

Cas9n-D10A

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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LT1_36_QUEEN-2m

Plasmid

#177708

Purpose

Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-QUEEN2m

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pOGG001

Plasmid

#113978

Purpose

Level 0 module. nptII promoter for constitutive gene expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

nptII promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSW002

Plasmid

#111248

Purpose

Broad host-range bacterial vector

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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iRFP-FRB-ER

Plasmid

#139317

Purpose

endoplasmic reticulum-resident FRB for chemically induced dimerization

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

iRFP:MTOR(2021-2113):[GGSA]2ILNSRV:SACM1L(521-587)

Expression

Mammalian

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-LacI-polylinker

Plasmid

#179271

Purpose

Cloning of GFP-LacI fusion proteins for LacI/LacO tethering

Depositor

Lienhard Schmitz

Article

Haas et al Front Cell Dev Biol. 2020 Sep 1;8:852. d

Type

Empty backbone

Tags

GFP and LacI

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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piGECInano-N1

Plasmid

#186190

Purpose

Expression of small FRET-based near-infrared genetically-encoded calcium indicator in mammalian cells

Depositor

Vladislav Verkhusha

Article

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pxpr_502-wt-ltr

Plasmid

#182647

Purpose

for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF, contains intact LTR

Depositor

Jakob Reiser

Article

Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

Insert

None

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pOGG202

Plasmid

#115503

Purpose

Clone of a level 1 unit which encodes IPTG-inducible sfGFP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

pL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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YN2_1_LT58_X2site

Plasmid

#177705

Purpose

Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-His-sfGFP

Plasmid

#180754

Purpose

pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Mutation

G140C- Please see depositor comments

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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