166,012 results

1. 2C-EGFP-t2a-mCD4

   Plasmid

   #216213

   Purpose

   Expresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoter

   Depositor

   Michelle Percharde

   Article

   Xie et al Genes Dev. 2022 Mar 1;36(5-6):331-347. d

   Insert

   CD4 (Cd4 Mouse)
   

   Use

   Tags

   GFP-T2A

   Expression

   Mammalian

   Mutation

   CD4 transmembrane domain only

   Promoter

   MERVL

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EGFP-Cyclin D3

   Plasmid

   #215052

   Purpose

   Expresses EGFP-Cyclin D3 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mU6-sgSik1_2nd-hU6-sgSik3_2nd

   Plasmid

   #177219

   Purpose

   Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase

   Depositor

   Monte Winslow

   Insert

   sgSik1_2nd/sgSik3_2nd
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6/hU6

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLyGo-Ec-1

   Plasmid

   #163127

   Purpose

   pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Ubiquitin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p663-UBC-miniTurbo-V5-DYRK4_IDG-K

   Plasmid

   #158184

   Purpose

   Gateway destination clone of DYRK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-DYRK4 (DYRK4 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available Since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-DIO-BACE-HA

   Plasmid

   #232353

   Purpose

   Beta-Secretase to be expressed (1:1) with either ATLAS protein.

   Depositor

   Don Arnold

   Article

   Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

   Insert

   BACE (BACE1 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMitoTimer

   Plasmid

   #52659

   Purpose

   Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo

   Depositor

   Zhen Yan

   Article

   Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1

   Insert

   DsRed1-E5
   

   Use

   Dsred

   Tags

   human cytochrome c oxidase subunit VIII mitochond…

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAVS1 pegRNA2 with trimmed attP

   Plasmid

   #222347

   Purpose

   pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   AAVS1 attP-installation pegRNA2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC gRNA Shuttle

   Plasmid

   #47024

   Purpose

   Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Insert

   gRNA Shuttle
   

   Use

   CRISPR; Cas9

   Tags

   Expression

   Plant

   Mutation

   G to T cloning mutation at position 323

   Promoter

   Medicago truncatula U6.6

   Available Since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLentiCMV_HyPer7RgDAAO

    Plasmid

    #217653

    Purpose

    Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    HyPer7 D amino acid oxidase
    

    Use

    Lentiviral

    Tags

    Nuclear export signal

    Expression

    Mammalian

    Mutation

    Fused DAAO to the C-terminus of HyPer7 using a Gl…

    Promoter

    CMV

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge