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pcDNA5/FRT/TO V5 DNAK

Plasmid

#19517

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

dnaK molecular chaperone DnaK

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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PA1024_pET21a(+)C-tag

Plasmid

#14579

Depositor

SeWon Suh

Article

Ha et al J Biol Chem. 2006 Jul 7. 281(27):18660-7

Insert

2-nitropropane dioxygenase (PA1024 Pseudomonas aeruginosa)

Tags

His

Expression

Bacterial

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only
pYSD095

Plasmid

#252985

Purpose

Encodes GFP11-M3 as a Type 4a part to be used for fluoresence complementation based detection in the yeast secretion/display (YSD2.0) MoClo toolkit Fluoresence Complementation

Depositor

Paul Young

Article

Jurić et al bioRxiv 2025.08.19.671047

Insert

GFP11-M3

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCDB24-XhoI_D40

Plasmid

#254737

Purpose

Design 40

Depositor

Brian Kuhlman

Article

Shelby et al Bioconjug Chem. 2026 Mar 17. doi: 10.102

Insert

Design 40

Tags

HIS and SUMO

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLann-HRV-3C

Plasmid

#254756

Purpose

Express Prescission Protease

Depositor

Brian Kuhlman

Article

Shelby et al Bioconjug Chem. 2026 Mar 17. doi: 10.102

Insert

HRV-3C

Tags

GST

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCDB24-XhoI_NF

Plasmid

#254741

Purpose

Naive Fusion

Depositor

Brian Kuhlman

Article

Shelby et al Bioconjug Chem. 2026 Mar 17. doi: 10.102

Insert

Naive Fusion

Tags

HIS and SUMO

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCDB24-XhoI_D42

Plasmid

#254738

Purpose

Design 42

Depositor

Brian Kuhlman

Article

Shelby et al Bioconjug Chem. 2026 Mar 17. doi: 10.102

Insert

Design 42

Tags

HIS and SUMO

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCDB24-XhoI_D12

Plasmid

#252874

Purpose

Design 12

Depositor

Brian Kuhlman

Article

Shelby et al Bioconjug Chem. 2026 Mar 17. doi: 10.102

Insert

Design 12

Tags

HIS and SUMO

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCDB24-XhoI_D48

Plasmid

#254739

Purpose

Design 48

Depositor

Brian Kuhlman

Article

Shelby et al Bioconjug Chem. 2026 Mar 17. doi: 10.102

Insert

Design 48

Tags

HIS and SUMO

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pET28g

Plasmid

#249630

Purpose

Clone genes for protein expression in Escherichia coli using Golden Gate cloning and the pET28a backbone

Depositor

Isabel Abreu

Article

Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi

Type

Empty backbone

Expression

Bacterial

Available Since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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puDNA5_SSF_MOR_2Ala_APEX2

Plasmid

#250760

Purpose

Mammalian cell expression of the mu opioid receptor with L387 and L390 mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

MOR(2Ala)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Mutation

L387 and L400 are mutated to alanines

Promoter

UBC

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5_TO_FRT_myc_GLUT4_2Ala_APEX2

Plasmid

#250767

Purpose

Mammalian cell expression of GLUT4 in which L500 and L503 are mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

GLUT4(2Ala)

Tags

APEX2 and myc (in exofacial loop)

Expression

Mammalian

Mutation

L500 and L503 mutated to alanines

Promoter

CMV

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_H1_VPS35_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP

Plasmid

#250765

Purpose

Mammalian cell expression of an shRNA against VPS35 and the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

VPS35 shRNA

Tags

SSF-SNAP(for MOR)

Expression

Mammalian

Promoter

H1 for shRNA, UBC for MOR

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_H1_Scramble_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP

Plasmid

#250764

Purpose

Mammalian cell expression of a scrambled shRNA and the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

Scrambled VPS35 shRNA

Tags

SSF-SNAP (for MOR)

Expression

Mammalian

Promoter

H1 for shRNA, UBC for MOR

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCambia2300-Aar1-ccdB-GmCH

Plasmid

#254405

Purpose

Gateway-compatible destination vector for bifluorescence complementation (BiFC). The plasmid carries Gus-mCherry expressed from the Arabidopsis Ubiquitin 10 promoter.

Depositor

Maria Harrison

Article

Ivanov et al Science. 2024 Jan 26;383(6681):443-448.

Type

Empty backbone

Expression

Plant

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_SSF_MOR_2Ala_APEX2

Plasmid

#250774

Purpose

Mammalian cell expression of the mu opioid receptor with L387 and L390 mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

MOR(2Ala)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Mutation

L387 and L400 are mutated to alanines

Promoter

UBC

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pN1_NUP62-EGFP3

Plasmid

#252748

Purpose

Expression of NUP62 with three tandem EGFPs.

Depositor

Kristen Verhey

Article

Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do

Insert

NUP62 (Nup62 Mouse)

Tags

E3xGFP

Expression

Mammalian

Available Since

April 3, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pE-SUMO_PspCas13b

Plasmid

#252295

Purpose

Expresses PspCas13b (WT) in bacterial cells.

Depositor

Hiroshi Nishimasu

Article

Ishikawa et al Nat Struct Mol Biol. 2025 Aug;32(8):1567

Insert

PspCas13b (WT)

Expression

Bacterial

Mutation

WT

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pLV hUBC-stuffer-mCherry-P2A-PuroR

Plasmid

#253231

Purpose

Backbone for insert expression with mCherry-2A-PuroR from lentiviral vector with cloning sites

Depositor

Charles Gersbach

Article

Reisman et al bioRxiv 2025.10.11.681828

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pNORg11-mScarlet-I

Plasmid

#249341

Purpose

Mediterraneibacter gnavus - Escherichia coli shuttle vector with mScarlet-I under the control of the constitutive promoter

Depositor

Nozomu Obana

Article

Obana et al Nat Commun. 2026 Mar 12. doi: 10.1038/s4

Inserts

cymR
veg promoter
CuO
mScarlet-I

Use

E. coli - r. gnavus shuttle vector

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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