Addgene

We narrowed to 1,224 results for: ADAP

Showing: 501 - 520 of 1224 results

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pET28a-Hook3 aa 1-160-GCN4

Plasmid

#74608

Purpose

Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Use

Tags

6x His, GCN4, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-JNKAR1-NES

Plasmid

#84632

Purpose

Unimolecular, FRET-based JNK activity reporter; cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ECFP-FHA1-JNKsubDD-Citrine-NES

Use

Tags

6xHis, Citrine, ECFP, Nuclear export signal (NES)…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean-WW-NES

Plasmid

#84629

Purpose

Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean-WW-NES

Use

Tags

6xHis, Cerulean, Nuclear export signal (NES), T7 …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shMki67

Plasmid

#160958

Purpose

Mki67 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Mki67 (Mki67 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDS2142

Plasmid

#184273

Purpose

Tet-off genome insertion system

Depositor

Dominique Sanglard

Article

Lemberg et al PLoS Pathog. 2022 Apr 11;18(4):e1010012.

Insert

CatTA

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 494-1318

Plasmid

#78587

Purpose

Expression flag-USP19 494-1318 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1318 (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-JNKsubDD-YPet-NES

Plasmid

#84633

Purpose

Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

JNKsubDD-YPet-NES

Use

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+1D

Plasmid

#149389

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

1bp inserted downstream of the IHFprox site

Promoter

pBAD

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shPrc1

Plasmid

#160959

Purpose

Prc1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Prc1 shRNA (Prc1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX4T2-Snf1-cat

Plasmid

#52683

Purpose

expression of the Snf1 kinase domain in bacteria

Depositor

Benjamin Turk

Article

Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

Insert

SNF1 (SNF1 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes kinase domain only, aa 41-315

Promoter

tac

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2-V660N

Plasmid

#12292

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I, H202Y and V660N; signal peptide (aa1-23) re…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA CrkII Y221F

Plasmid

#50732

Purpose

mammalian expression of CrkII Y221F mutant

Depositor

Kenneth Yamada

Article

Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

Insert

CrkII Y221F (CRK Human)

Use

Tags

Expression

Mammalian

Mutation

Y221F

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2-P681H

Plasmid

#12290

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

A74V, M82I, H202Y and P681H; signal peptide (aa1-…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pET28a-Hook3 aa 1-239

Plasmid

#74609

Purpose

Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239 (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Ets2DBM

Plasmid

#28129

Depositor

Pumin Zhang

Article

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

Ets2 (ETS2 Human)

Use

Mouse Targeting and Retroviral

Tags

Expression

Mammalian

Mutation

The putative destruction box (RGTL) residues 20–2…

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-ERKsubDD-Venus-NES

Plasmid

#84630

Purpose

Encodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ERKsubDD-Venus-NES

Use

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK-flag-USP19 494-1290

Plasmid

#78580

Purpose

Expression flag-USP19 494-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1290 (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493 and mutated 1291 residue…

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pET28a-Hook3 aa 1-160

Plasmid

#74607

Purpose

Expresses Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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EC.SurA.pTYB1.a21-281&390-428.L239R

Plasmid

#15959

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

Escherichia coli surA delta PPIase domain 2

Use

Tags

intein

Expression

Bacterial

Mutation

residues 282-389 deleted; L239R mutation

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pHInt-AppA-KanR-loxP

Plasmid

#232910

Purpose

Plasmid for marker free integration of E. coli appA at the phac1 locus of Cupriavidus necator.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

AppA (appA )

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Pj5

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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