We narrowed to 25,911 results for: TIM

1. FHL-cpmTq2-Calcium-lifetime-sensor

   Plasmid

   #129628

   Purpose

   Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   FHL-cpmTq2-Calcium-lifetime-sensor
   

   Tags

   6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

   Expression

   Bacterial and Mammalian

   Promoter

   CMV,&Rhamnose

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lck-cpmTq2-Calcium-lifetime-sensor

   Plasmid

   #129627

   Purpose

   Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   Lck-cpmTq2-Calcium-lifetime-sensor
   

   Tags

   Lck

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Str-STIM1-NN_ST-SBP-mCherry

   Plasmid

   #65263

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xnls-cpmTq2-Calcium-lifetime-sensor

   Plasmid

   #129626

   Purpose

   Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   3xnls-cpmTq2-Calcium-lifetime-sensor
   

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-TNT-CFP-STIM1-C227W

   Plasmid

   #68403

   Purpose

   Expresses a STIM1 gain-of-function mutant in mammalian cells

   Depositor

   Yubin Zhou

   Article

   Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

   Insert

   CFP-STIM1 (STIM1 Human)
   

   Tags

   Cyan Fluorescent Protein (CFP)

   Expression

   Mammalian

   Mutation

   Changed Cysteine 227 to Tryptophan; CFP was inser…

   Promoter

   CMV

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

6. mNeongreen-pGBD(PKN1 codon optimized)

   Plasmid

   #129634

   Purpose

   Visualization of active RhoA

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

   Insert

   RhoA binding doamin of PKN1(30 - 100) codon optimized
   

   Expression

   Mammalian

   Promoter

   CMVdel

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNK036C_AttB_mkozak_mGreenLantern-STIM1_IRES_mCherry-H2A-P2A-PuroR

   Plasmid

   #232938

   Purpose

   Low expression plasmid of STIM1 with N-terminal mGreenLantern tag for Matreyek Bxb1 (GT) landing pad

   Depositor

   Kenneth Matreyek

   Article

   Kamath et al Genetics. 2025 Jul 30:iyaf146. doi: 10.1

   Insert

   STIM1 (STIM1 Human)
   

   Tags

   mGreenLantern

   Expression

   Mammalian

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-15b_T. maritima His-tRNase Z

   Plasmid

   #242436

   Purpose

   Expresses T. maritima tRNase Z in E. coli.

   Depositor

   Sebastian Maerkl

   Article

   Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

   Insert

   tRNase Z
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM-60-HsAGO2-448-859-optim_K

   Plasmid

   #146729

   Purpose

   Bacterial Expression of HsAGO2_448-859-optim

   Depositor

   Elisa Izaurralde

   Insert

   HsAGO2_448-859-optim (AGO2 Human)
   

   Expression

   Bacterial

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-ZmIAA29 (yeast codon optimized)

    Plasmid

    #188441

    Purpose

    Zea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimization

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    Auxin Signaling Repressor IAA29 (yeast codon optimized)
    

    Use

    Cloning

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIG-732pUC19-tNGFR-P2A-STIM1

    Plasmid

    #186119

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    STIM1 (STIM1 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. px458-AMBRA1 (optimized for N-terminal knock-in)

    Plasmid

    #172608

    Purpose

    Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against AMBRA1 (AMBRA1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONRPEX18Km-SceI-pheS

    Plasmid

    #111610

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Available Since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDAI-SceI-pheS

    Plasmid

    #111611

    Purpose

    Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Insert

    pheS
    

    Expression

    Bacterial

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCdrA-gfp(ASV)C

    Plasmid

    #111615

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Expression

    Bacterial

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCdrA-gfpC

    Plasmid

    #111614

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Expression

    Bacterial

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTn7CdrA-gfp(ASV)C

    Plasmid

    #111617

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Expression

    Bacterial

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTn7CdrA-gfpC

    Plasmid

    #111616

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Expression

    Bacterial

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONRPEX18Tp-SceI-pheS

    Plasmid

    #111608

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. miniTn7(Gm)PA1/04/03-eyfp-a

    Plasmid

    #111620

    Purpose

    eYFP Fluorescent tagging of bacteria at their Tn7-site

    Depositor

    Tim Tolker-Nielsen

    Article

    Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

    Insert

    PA1/04/03-eyfp
    

    Expression

    Bacterial

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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