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pTwist-SARS-CoV-2 Spike-deltaC18 S477N (AAC) T478K (AAA)

Plasmid

#212470

Purpose

Encodes SARS-CoV-2 spike S477N, T478K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike S477N, T478K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

S477N, T478K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 S477N (AAC) T478K (AAA)

Plasmid

#212470

Purpose

Encodes SARS-CoV-2 spike S477N, T478K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike S477N, T478K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

S477N, T478K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 L452R (CGT) T478K (AAA)

Plasmid

#212437

Purpose

Encodes SARS-CoV-2 spike L452R, T478K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L452R, T478K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L452R, T478K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 L452R (CGT) T478K (AAA)

Plasmid

#212437

Purpose

Encodes SARS-CoV-2 spike L452R, T478K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L452R, T478K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L452R, T478K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 K417T (ACG) E484K (AAA)

Plasmid

#212382

Purpose

Encodes SARS-CoV-2 spike E484K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E484K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 K417T (ACG) E484K (AAA)

Plasmid

#212382

Purpose

Encodes SARS-CoV-2 spike E484K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E484K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT) E484K (AAA)

Plasmid

#212352

Purpose

Encodes SARS-CoV-2 spike K417N, E4848K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike K417N, E4848K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

K417N, E4848K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 E484K (AAA) N501Y (TAT)

Plasmid

#212354

Purpose

Encodes SARS-CoV-2 spike E484K, N501Y for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484K, N501Y (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E484K, N501Y

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT) E484K (AAA)

Plasmid

#212352

Purpose

Encodes SARS-CoV-2 spike K417N, E4848K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike K417N, E4848K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

K417N, E4848K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 E484K (AAA) N501Y (TAT)

Plasmid

#212354

Purpose

Encodes SARS-CoV-2 spike E484K, N501Y for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484K, N501Y (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E484K, N501Y

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

Plasmid

#47542

Purpose

engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

Depositor

Henry Lester

Article

Frazier et al J Biol Chem. 2013 May 29.

Insert

Glutamate-gated chloride channels

Expression

Mammalian

Mutation

retention motif in ER (starting at R340) changed …

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

Plasmid

#47542

Purpose

engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

Depositor

Henry Lester

Article

Frazier et al J Biol Chem. 2013 May 29.

Insert

Glutamate-gated chloride channels

Expression

Mammalian

Mutation

retention motif in ER (starting at R340) changed …

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 S477N (AAC) T478K (AAA) E484A (GCA)

Plasmid

#212472

Purpose

Encodes SARS-CoV-2 spike S477N, T478K, E484A for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike S477N, T478K, E484A (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

S477N, T478K, E484A

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 S477N (AAC) T478K (AAA) E484A (GCA)

Plasmid

#212472

Purpose

Encodes SARS-CoV-2 spike S477N, T478K, E484A for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike S477N, T478K, E484A (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

S477N, T478K, E484A

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT) L452R (CGT) T478K (AAA)

Plasmid

#212438

Purpose

Encodes SARS-CoV-2 spike K417N, L452R, T478K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike K417N, L452R, T478K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

K417N, L452R, T478K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT) L452R (CGT) T478K (AAA)

Plasmid

#212438

Purpose

Encodes SARS-CoV-2 spike K417N, L452R, T478K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike K417N, L452R, T478K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

K417N, L452R, T478K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS425-M25-Afg1

Plasmid

#61720

Purpose

yeast Afg1 AAA protein in yeast expression vector

Depositor

Oleh Khalimonchuk

Article

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

Afg1 (AFG1 Budding Yeast)

Expression

Yeast

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only
pRS425-M25-Afg1

Plasmid

#61720

Purpose

yeast Afg1 AAA protein in yeast expression vector

Depositor

Oleh Khalimonchuk

Article

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

Afg1 (AFG1 Budding Yeast)

Expression

Yeast

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only
pET151-Vps4(101-437)-Hcp1

Plasmid

#87737

Purpose

Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Tags

6xHis-V5-TEV and Hcp1

Expression

Bacterial

Mutation

deleted amino acids 1-100

Promoter

T7

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-Vps4(101-437)-Hcp1

Plasmid

#87737

Purpose

Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Tags

6xHis-V5-TEV and Hcp1

Expression

Bacterial

Mutation

deleted amino acids 1-100

Promoter

T7

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge