We narrowed to 2,346 results for: puc19

1. pLinker-BirA-3xHA-HXGPRT-loxP

   Plasmid

   #86668

   Purpose

   for generation of BirA tagged strains using CRISPR tagging technology

   Depositor

   David Sibley

   Insert

   Linker-BirA-3xHA-HXGPRT 3'UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUPRT::Floxed DHFR-TS*

   Plasmid

   #100606

   Purpose

   TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.

   Depositor

   David Sibley

   Insert

   TgUPRT::Floxed TgDHFR-TS*
   

   Use

   Toxoplasma expression

   Tags

   Expression

   Mutation

   Pyrimethamine resistance

   Promoter

   pTgDHFR-TS

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZFX-donor

   Plasmid

   #112366

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZFX with GFP

   Depositor

   Kevin White

   Insert

   ZFX homology arms flanking EGFP-IRES-Neo cassette (ZFX Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS18

   Plasmid

   #225681

   Purpose

   Confers puromycin resistance to Salpingoeca rosetta

   Depositor

   Thibaut Brunet

   Article

   Combredet et al bioRxiv 2024.07.13.603360

   Insert

   pEFL-Pac-Actin_3'UTR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDD288

   Plasmid

   #137017

   Purpose

   mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Daniel Dickinson, Bob Goldstein

   Insert

   mNG-GLO^SEC^3xFlag + ccdB
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans GLO-optimized mNG

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPITX2-donor

   Plasmid

   #176372

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   PITX2 (PITX2 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDD291

   Plasmid

   #137019

   Purpose

   mKate2-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Daniel Dickinson, Bob Goldstein

   Insert

   mKate2-GLO^SEC^3xFlag + ccdB
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans GLO-optimized mKate2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZBTB5-donor

   Plasmid

   #112303

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZBTB5 with GFP

   Depositor

   Kevin White

   Insert

   ZBTB5 homology arms flanking EGFP-IRES-Neo cassette (ZBTB5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT643

   Plasmid

   #8610

   Depositor

   Robert Hartley

   Article

   Hartley et al Biochemistry 1993 Jun 15;32(23):5978-84.

   Insert

   barstar A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Two cysteines of barstar changed to alanine, C40A…

   Promoter

   Available since

   Jan. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDD316

    Plasmid

    #137020

    Purpose

    mTurq2-GLO^SEC^2xHA vector with ccdB markers for cloning homology arms

    Depositor

    Daniel Dickinson, Bob Goldstein

    Insert

    mTurq2-GLO^SEC^2xHA + ccdB
    

    Use

    CRISPR and Cre/Lox

    Tags

    2xHA and C. elegans GLO-optimized mTurq2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAMTA2-donor

    Plasmid

    #132501

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    CAMTA2 (CAMTA2 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    none

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDD289

    Plasmid

    #137018

    Purpose

    GFP-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Daniel Dickinson, Bob Goldstein

    Insert

    GFP-GLO^SEC^3xFlag + ccdB
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans GLO-optimized GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZSCAN18-donor

    Plasmid

    #113821

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZSCAN18 with GFP

    Depositor

    Kevin White

    Insert

    ZSCAN18 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN18 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZNF561-donor

    Plasmid

    #113801

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF561 with GFP

    Depositor

    Kevin White

    Insert

    ZNF561 homology arms flanking EGFP-IRES-Neo cassette (ZNF561 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZNF700-donor

    Plasmid

    #112385

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF700 with GFP

    Depositor

    Kevin White

    Insert

    ZNF700 homology arms flanking EGFP-IRES-Neo cassette (ZNF700 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pZNF169-donor

    Plasmid

    #112376

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF169 with GFP

    Depositor

    Kevin White

    Insert

    ZNF169 homology arms flanking EGFP-IRES-Neo cassette (ZNF169 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZFP1-donor

    Plasmid

    #112374

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZFP1 with GFP

    Depositor

    Kevin White

    Insert

    ZFP1 homology arms flanking EGFP-IRES-Neo cassette (ZFP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZNF227-donor

    Plasmid

    #112368

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF227 with GFP

    Depositor

    Kevin White

    Insert

    ZNF227 homology arms flanking EGFP-IRES-Neo cassette (ZNF227 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOTX1-donor

    Plasmid

    #112364

    Purpose

    CRISPR donor plasmid to tag human transcription factor OTX1 with GFP

    Depositor

    Kevin White

    Insert

    OTX1 homology arms flanking EGFP-IRES-Neo cassette (OTX1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZNF175-donor

    Plasmid

    #112347

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF175 with GFP

    Depositor

    Kevin White

    Insert

    ZNF175 homology arms flanking EGFP-IRES-Neo cassette (ZNF175 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge